Functional redundancy of two C. elegans homologs of the histone chaperone Asf1 in germline DNA replication
| Autor: | Iwen F. Grigsby, Christine A. Morton, Eric M. Rutledge, Fern P. Finger |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA Replication
Male Embryo Nonmammalian asfl-1 Somatic cell Mutant Disorders of Sex Development Replication Biology Models Biological Gametogenesis Germline Histones 03 medical and health sciences 0302 clinical medicine Oogenesis Asf1 Sterility Animals RNA Messenger Caenorhabditis elegans Caenorhabditis elegans Proteins Spermatogenesis Gene Molecular Biology Genes Helminth In Situ Hybridization 030304 developmental biology Genetics 0303 health sciences fungi DNA replication Helminth Proteins Cell Biology biology.organism_classification Chromatin Meiosis Histone unc-85 C. elegans biology.protein Female 030217 neurology & neurosurgery Molecular Chaperones Developmental Biology |
| Zdroj: | Developmental Biology. 329(1):64-79 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2009.02.015 |
| Popis: | Eukaryotic genomes contain either one or two genes encoding homologs of the highly conserved histone chaperone Asf1, however, little is known of their in vivo roles in animal development. UNC-85 is one of the two Caenorhabditis elegans Asf1 homologs and functions in post-embryonic replication in neuroblasts. Although UNC-85 is broadly expressed in replicating cells, the specificity of the mutant phenotype suggested possible redundancy with the second C. elegans Asf1 homolog, ASFL-1. The asfl-1 mRNA is expressed in the meiotic region of the germline, and mutants in either Asf1 genes have reduced brood sizes and low penetrance defects in gametogenesis. The asfl-1, unc-85 double mutants are sterile, displaying defects in oogenesis and spermatogenesis, and analysis of DNA synthesis revealed that DNA replication in the germline is blocked. Analysis of somatic phenotypes previously observed in unc-85 mutants revealed that they are neither observed in asfl-1 mutants, nor enhanced in the double mutants, with the exception of enhanced male tail abnormalities in the double mutants. These results suggest that the two Asf1 homologs have partially overlapping functions in the germline, while UNC-85 is primarily responsible for several Asf1 functions in somatic cells, and is more generally involved in replication throughout development. |
| Databáze: | OpenAIRE |
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