The yeast protein mrs6p, a homologue of the RabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions
| Autor: | Rudolf J. Schweyen, Martin Waldherr, A Voskova, Antonella Ragnini, R Teply |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Cytoplasm
Choroideraemia Saccharomyces cerevisiae Proteins GTPase-activating protein G protein Mutant Saccharomyces cerevisiae Genes Fungal Molecular Sequence Data Small G Protein Biology yeast Gene product Fungal Proteins genomic GTP-Binding Proteins Rab escort protein Genetics Humans Amino Acid Sequence Settore BIO/10 DNA Fungal Genes Suppressor Adaptor Proteins Signal Transducing Guanine Nucleotide Dissociation Inhibitors Choroideraemia Rab escort protein protein trafficking genomic yeast Alkyl and Aryl Transferases Base Sequence General Medicine biology.organism_classification Cell biology Mitochondria Vesicular transport protein Phenotype rab GTP-Binding Proteins Rab protein trafficking Carrier Proteins |
| Popis: | MRS6 is a newly-identified gene in the yeast Saccharomyces cerevisiae. Its product Mrs6p shows significant homology to the mammalian GDP dissociation inhibitor (GDI) of Rab/Ypt-type small G proteins and to the human choroideraemia protein (CHM), the component A of Rab-specific GGTase II. The interaction of Mrs6p with G proteins is indicated by our observation that the MRS6 gene suppresses the effect of a temperature-sensitive ypt1 mutation. Disruption of the MRS6 gene is lethal to haploid yeast cells. This is consistent with the notion that Mrs6p is interacting with Rab/Ypt-type small G proteins, which are known to have essential functions in vesicular transport. Unexpectedly, the MRS6 gene product also affects mitochondrial functions as revealed by the facts that high-copy numbers of MRS6 (1) suppress the pet- phenotype of mrs2-1 mutant strains and (2) cause a weak pet- phenotype in wild-type strains. We conclude from these results that the MRS6 gene product has a vital function in connection with Rab/Ypt-type proteins in the cytoplasm and, in addition, affects mitochondrial functions. |
| Databáze: | OpenAIRE |
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