Alternatively spliced CD44 isoforms containing exon v10 promote cellular adhesion through the recognition of chondroitin sulfate-modified CD44
| Autor: | Roland K. Chiu, G J Dougherty, Shona T. Dougherty, Armin Droll, Carmine Carpenito, David L. Cooper |
|---|---|
| Přispěvatelé: | Department of Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
DNA Complementary Cell Line Exon chemistry.chemical_compound Adjuvants Immunologic Immunologic Complementary Cell Adhesion Journal Article Animals Protein Isoforms Chondroitin sulfate Adjuvants Hyaluronic Acid Cell adhesion Cell Line Transformed COS cells biology Alternative splicing CD44 Chondroitin Sulfates Cell Biology Adhesion DNA Exons Molecular biology Alternative Splicing Hyaluronan Receptors chemistry Transformed COS Cells biology.protein |
| Zdroj: | Experimental Cell Research, 248(1), 314-21. HANLEY & BELFUS-ELSEVIER INC |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.1999.4391 |
| Popis: | Correlations have been noted between the expression of certain alternatively spliced CD44 isoforms and the metastatic propensity of various histologically distinct tumor cell types. The precise mechanism by which particular CD44 isoforms contribute to the metastatic process is, however, unclear. In the present study we demonstrate that CD44R2, a CD44 isoform highly expressed on activated and transformed hemopoietic cells, can recognize and bind a common determinant present on CD44H and CD44R1. Importantly, CD44H lacked this activity. Pretreatment of TIL1 cells expressing CD44H or CD44R1 with chondroitinase ABC inhibited adhesion to CD44R2, suggesting that the unique inserted region present within the CD44R2 molecule, encoded by exon v10, mediates cell adhesion by potentiating the recognition of chondroitin sulfate moieties presented in association with other CD44 molecules. These data help explain the differential involvement of v10-containing CD44 isoforms in tumor metastasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect