| Autor: |
Weixian Deng, Jihui Sha, Fanglei Xue, Yasaman Jami-Alahmadi, Kathrin Plath, James Wohlschlegel |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Analytical chemistry, vol 94, iss 46 |
| ISSN: |
1520-6882 |
| Popis: |
High-field asymmetric waveform ion mobility spectrometry (FAIMS) enables gas-phase separations on a chromatographic time scale and has become a useful tool for proteomic applications. Despite its emerging utility, however, the molecular determinants underlying peptide separation by FAIMS have not been systematically investigated. Here, we characterize peptide transmission in a FAIMS device across a broad range of compensation voltages (CVs) and used machine learning to identify charge state and three-dimensional (3D) electrostatic peptide potential as major contributors to peptide intensity at a given CV. We also demonstrate that the machine learning model can be used to predict optimized CV values for peptides, which significantly improves parallel reaction monitoring workflows. Together, these data provide insight into peptide separation by FAIMS and highlight its utility in targeted proteomic applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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