Elevated OCT1 participates in colon tumorigenesis and independently predicts poor prognoses of colorectal cancer patients

Autor: Chao Li, Chao Xiao, Yupeng Wang, Lin Zhong, Ying ming Xue, Guo he Song, Xing Sun, Hua mei Tang, Gui long Deng, Zhaowen Wang, Fu dong Yu, Zhi hai Peng, Jian Chen, Xiaoliang Wang
Jazyk: angličtina
Rok vydání: 2015
Předmět:
0301 basic medicine
Oncology
Male
Cancer Research
Colorectal cancer
Proliferation
Kaplan-Meier Estimate
Bioinformatics
OCT1
Stage (cooking)
Octamer transcription factor
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
Middle Aged
Prognosis
Immunohistochemistry
Up-Regulation
Gene Expression Regulation
Neoplastic
Cell Transformation
Neoplastic
Original Article
Female
RNA Interference
Colorectal Neoplasms
medicine.medical_specialty
Colon
Transplantation
Heterologous
Mice
Nude
03 medical and health sciences
In vivo
Predictive Value of Tests
Internal medicine
Cell Line
Tumor
medicine
Biomarkers
Tumor
Animals
Humans
Aged
Cell Proliferation
Proportional hazards model
Cell growth
business.industry
Cancer
medicine.disease
HCT116 Cells
030104 developmental biology
Multivariate Analysis
business
Octamer Transcription Factor-1
Zdroj: Tumour Biology
ISSN: 1423-0380
1010-4283
Popis: Octamer transcription factor 1 (OCT1) was found to influence the genesis and progression of numerous cancers except for colorectal cancer (CRC). This study tried to explore the role of OCT1 in CRC and clarify the association between its expression and patients’ clinical outcome. Transcriptional and post-transcriptional expression of OCT1 was detected in CRC cancerous tissues and paired normal mucosae by real-time PCR as well as immunohistochemistry. Moreover, the effect of OCT1 knockdown on CRC cell proliferation was investigated both in vitro and in vivo using Cell Counting Kit-8 assay, colony-forming assay, and mouse tumorigenicity assay. Expression of OCT1 was found to be elevated in CRC. Suppression of OCT1 significantly inhibited CRC cell proliferation both in vitro and in vivo. Furthermore, upregulated level of OCT1 was significantly associated with N stage, M stage, and American Joint Committee on Cancer (AJCC) stage (P = 0.027, 0.014, and 0.002, respectively) as well as differential degree (P = 0.022). By using multivariate Cox hazard model, OCT1 was also shown to be a factor independently predicting overall survival (OS; P = 0.013, hazard ratio = 2.747, 95 % confidence interval 1.125 to 3.715) and disease-free survival (DFS; P = 0.004, hazard ratio = 2.756, 95 % confidence interval 1.191 to 4.589) for CRC patients. Our data indicate that OCT1 carries weight in colorectal carcinogenesis and functions as a novel prognostic indicator and a promising target of anti-cancer therapy for CRC.
Databáze: OpenAIRE
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