Highly Charged Ru(II) Polypyridyl Complexes as Photosensitizer Agents in Photodynamic Therapy of Epithelial Ovarian Cancer Cells

Autor:	Luca Conti, Gina Elena Giacomazzo, Barbara Valtancoli, Mauro Perfetti, Alberto Privitera, Claudia Giorgi, Patrick Severin Sfragano, Ilaria Palchetti, Sara Pecchioli, Paola Bruni, Francesca Cencetti
Rok vydání:	2022
Předmět:	Ovarian Neoplasms Photosensitizing Agents Organic Chemistry Antineoplastic Agents General Medicine Carcinoma Ovarian Epithelial Catalysis Ruthenium Computer Science Applications Inorganic Chemistry Photochemotherapy Coordination Complexes Cell Line Tumor Humans Female Physical and Theoretical Chemistry Neoplasm Recurrence Local Reactive Oxygen Species drug discovery coordination complexes phototoxicity reactive oxygen species Molecular Biology Spectroscopy HeLa Cells
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 13302
ISSN:	1422-0067
Popis:	Ovarian cancer recurrence is frequent and associated with chemoresistance, leading to extremely poor prognosis. Herein, we explored the potential anti-cancer effect of a series of highly charged Ru(II)-polypyridyl complexes as photosensitizers in photodynamic therapy (PDT), which were able to efficiently sensitize the formation of singlet oxygen upon irradiation (Ru12+ and Ru22+) and to produce reactive oxygen species (ROS) in their corresponding dinuclear metal complexes with the Fenton active Cu(II) ion/s ([CuRu1]4+ and [Cu2Ru2]6+). Their cytotoxic and anti-tumor effects were evaluated on human ovarian cancer A2780 cells both in the absence or presence of photoirradiation, respectively. All the compounds tested were well tolerated under dark conditions, whereas they switched to exert anti-tumor activity following photoirradiation. The specific effect was mediated by the onset of programed cell death, but only in the case of Ru12+ and Ru22+ was preceded by the loss of mitochondrial membrane potential soon after photoactivation and ROS production, thus supporting the occurrence of apoptosis via type II photochemical reactions. Thus, Ru(II)-polypyridyl-based photosensitizers represent challenging tools to be further investigated in the identification of new therapeutic approaches to overcome the innate chemoresistance to platinum derivatives of some ovarian epithelial cancers and to find innovative drugs for recurrent ovarian cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5cc0b4751d2c886defc64cf0eff0afe5 https://pubmed.ncbi.nlm.nih.gov/36362089 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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