Distinct Polysaccharide Utilization Profiles of Human Intestinal Prevotella copri Isolates
| Autor: | Hannah Fehlner-Peach, Claire Gottsegen, Varsha Raghavan, Dan R. Littman, Jose U. Scher, Cara Magnabosco, John D. Wiltshire-Gordon, Nicola Segata, Laura M. Cox, Richard Bonneau, Adrian Tett, Aaron Watters |
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| Rok vydání: | 2019 |
| Předmět: |
Prevotella
Biology Microbiology Article 03 medical and health sciences 0302 clinical medicine Polysaccharides Virology Humans Clade Gene 030304 developmental biology Whole genome sequencing Genetics 0303 health sciences Strain (biology) Human microbiome Genetic Variation Plants Diet Gastrointestinal Microbiome Intestines Metagenomics Polysaccharide transport Parasitology Genome Bacterial 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | Cell Host & Microbe Cell Host Microbe |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2019.10.013 |
| Popis: | Summary Gut-dwelling Prevotella copri (P. copri), the most prevalent Prevotella species in the human gut, have been associated with diet and disease. However, our understanding of their diversity and function remains rudimentary because studies have been limited to 16S and metagenomic surveys and experiments using a single type strain. Here, we describe the genomic diversity of 83 P. copri isolates from 11 human donors. We demonstrate that genomically distinct isolates, which can be categorized into different P. copri complex clades, utilize defined sets of polysaccharides. These differences are exemplified by variations in susC genes involved in polysaccharide transport as well as polysaccharide utilization loci (PULs) that were predicted in part from genomic and metagenomic data. Functional validation of these PULs showed that P. copri isolates utilize distinct sets of polysaccharides from dietary plant, but not animal, sources. These findings reveal both genomic and functional differences in polysaccharide utilization across human intestinal P. copri strains. |
| Databáze: | OpenAIRE |
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