Acetylcholinesterase Inhibitors among Zingiber officinale Terpenes—Extraction Conditions and Thin Layer Chromatography-Based Bioautography Studies
| Autor: | Agnieszka Ludwiczuk, Zbigniew Marzec, Lidia Czernicka, Wirginia Kukula-Koch, Edward Rój, Agata Jarzab |
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| Rok vydání: | 2020 |
| Předmět: |
Monoterpene
Pharmaceutical Science extraction optimization 01 natural sciences Analytical Chemistry lcsh:QD241-441 Terpene Matrix (chemical analysis) chemistry.chemical_compound lcsh:Organic chemistry Zingiberaceae Drug Discovery TLC bioautography Zingiber officinale Physical and Theoretical Chemistry thin layer chromatography-high-performance liquid chromatography-mass spectrometry Chromatography quantitative analysis 010405 organic chemistry Chemistry 010401 analytical chemistry Organic Chemistry Extraction (chemistry) neurodegeneration gas chromatography-mass spectrometry Thin-layer chromatography 0104 chemical sciences acetylcholinesterase inhibitors Chemistry (miscellaneous) Molecular Medicine Diethyl ether Gas chromatography–mass spectrometry terpenes |
| Zdroj: | Molecules Volume 25 Issue 7 Molecules, Vol 25, Iss 1643, p 1643 (2020) |
| ISSN: | 1420-3049 |
| Popis: | Although numerous studies have been conducted on ginger extracts and fractions, the data on the pharmacological activity of single constituents of Zingiber officinale are still insufficient. To assess the antidementia properties of the plant, a thin layer chromatography (TLC)-based bioautography acetylcholinesterase inhibitory assay was performed on the Zingiber officinale diethyl ether extract. It led to the recognition of three active inhibitors among volatile constituents of the plant: ar-curcumene (A), &alpha sesquiphellandrene (B) and a-zingiberene (C). The identification of the components was possible thanks to the application of a TLC&ndash HPLC-MS interface analysis of active zones and the GC-MS qualitative analysis of the tested samples. Based on the obtained results, the influence of several extraction techniques (hydrodistillation&mdash HD, pressurized liquid extraction or accelerated solvent extraction&mdash ASE, shaking maceration&ndash SM, supercritical fluid extraction&ndash SFE, and ultrasound-assisted extraction&mdash UAE) on the recovery of the active metabolites from plant material was assessed to deliver enriched extracts. As a result, HD and SFE, were found to be the most efficient methods to recover the volatile components and the concentrations of A, B, and C reached 0.51 ± 0.025, 0.77 ± 0.045, and 1.67 ± 0.11 percent, respectively. Only HD and SFE were found to recover monoterpene hydrocarbons from the plant matrix. The remaining techniques provided extracts rich in more complex constituents, like sesquiterpenes. |
| Databáze: | OpenAIRE |
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