Downregulation of microRNA-382 is associated with poor outcome of esophageal squamous cell carcinoma

Autor: Bo Qi, Ting-Min Chang, Han-Chen Li, Tian-Yun Wang, Baosheng Zhao, Jian-Guo Lu, Xiuguang Qin, Shang-Guo Liu, Yuzhen Liu, Ying-Hua Ji, Wen-Jian Yao
Rok vydání: 2015
Předmět:
Male
Oncology
medicine.medical_specialty
Time Factors
Esophageal Neoplasms
medicine.medical_treatment
Down-Regulation
Kaplan-Meier Estimate
Esophageal squamous cell carcinoma
Disease-Free Survival
Metastasis
Downregulation and upregulation
Risk Factors
Internal medicine
microRNA
Biomarkers
Tumor
medicine
Humans
neoplasms
Aged
Neoplasm Staging
Proportional Hazards Models
Proportional hazards model
business.industry
Gastroenterology
General Medicine
Basic Study
Middle Aged
Esophageal cancer
medicine.disease
digestive system diseases
Surgery
Esophagectomy
Gene Expression Regulation
Neoplastic
stomatognathic diseases
MicroRNAs
Treatment Outcome
Real-time polymerase chain reaction
Chemotherapy
Adjuvant
Carcinoma
Squamous Cell
Disease Progression
Female
Esophageal Squamous Cell Carcinoma
Neoplasm Recurrence
Local
business
Zdroj: World Journal of Gastroenterology. 21:6884-6891
ISSN: 1007-9327
DOI: 10.3748/wjg.v21.i22.6884
Popis: AIM: To study the potential prognostic role of microRNA-382 (miR-382) in esophageal squamous cell carcinoma (ESCC). METHODS: Forty six patients were divided into 2 groups according to postoperative survival time: the poor outcome group (28 patients), who showed early metastasis but no recurrence, and died within 1 year after surgery, 12 patients of the group received postoperative chemotherapy treatment that was given after early metastasis happening; the good outcome group (18 patients), who had no clinical metastasis and recurrence, and survived 5 years or more after surgery, all patients did not receive any postoperative treatment. Total RNA was extracted from the patients’ formalin-fixed and paraffin-embedded esophageal cancer tissues. miR-382 level was evaluated using high-throughput real-time quantitative polymerase chain reaction analysis. The correlation between miR-382 level and clinicopathologic features was analyzed through COX regression model, and Kaplan-Meier analysis was used to analyze the relationship between miR-382 level and patient survival time. RESULTS: miR-382 was differentially expressed in the two groups. Overall the average miR-382 level in the ESCC patients with good outcome was 9.8 ± 3.8, while miR-382 level in the ESCC patients with poor outcome was 3.0 ± 0.8. The differences of miR-382 levels between two groups were significant (P < 0.05). Kaplan-Meier analysis results showed that miR-382 expression level generally had a significant reverse-correlation with ESCC patient survival time (P < 0.001), in which the patients with higher expressions of miR-382 had a longer survival time either among individuals with the same tumor stage or among the overall patients. CONCLUSION: miR-382 levels are reverse-correlated with ESCC poor outcomes, suggesting that miR-382 could be a potential predictive biomarker for both prognosis and treatment of ESCC.
Databáze: OpenAIRE
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