Addition of beta-mercaptoethanol or Trolox at the morula/blastocyst stage improves the quality of bovine blastocysts and prevents induction of apoptosis and degeneration by prooxidant agents
| Autor: | Rudolf De Roover, S. Leonard, Jean-Magloire Feugang, André Moens, Isabelle Donnay, Franz Dessy |
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| Rok vydání: | 2003 |
| Předmět: |
Time Factors
medicine.medical_treatment Apoptosis Biology medicine.disease_cause Morula Antioxidants Andrology chemistry.chemical_compound Food Animals Culture Techniques medicine In Situ Nick-End Labeling Animals Blastocyst Chromans Small Animals reproductive and urinary physiology Mercaptoethanol Dose-Response Relationship Drug Ethanol Equine Vitamin E Embryo Embryo culture Oxidants Glutathione In vitro Oxidative Stress medicine.anatomical_structure Biochemistry chemistry embryonic structures Animal Science and Zoology Cattle Female Trolox Oxidation-Reduction Oxidative stress |
| Zdroj: | Theriogenology. 61(1) |
| ISSN: | 0093-691X |
| Popis: | This study was conducted to evaluate the effect of beta-mercaptoethanol (a stimulator of glutathione synthesis) and Trolox (an hydrosoluble analogue of Vitamin E) on bovine embryos cultured from the morula stage (Day 5 post-insemination; pi) under oxidative stress conditions. Culture of embryos with increased doses of Trolox showed a dose-dependent embryotoxicity on Day 8 pi. The use of 400 microM Trolox as well as beta-mercaptoethanol at 100 microM prevented at least partly (P < 0.05) the prooxidant-induced blastocyst degeneration on Day 8. Hatching rates of surviving blastocysts were significantly increased by both antioxidants and beta-mercaptoethanol alone improved their mean cell numbers, which was significant in the ICM (P < 0.05). Analysis of their effect on Day 7 pi showed that both the antioxidants significantly reduced the prooxidant-induced apoptosis and beta-mercaptoethanol diminished the physiological level of apoptosis as well as it stimulated the glutathione synthesis (P < 0.05). In addition, a comparison between in vitro- and in vivo-produced embryos showed that the levels of apoptosis were similar at the same age post-insemination (morulae and blastocysts) but increased steadily with the embryonic age in in vitro ones.In conclusion, beta-mercaptoethanol and Trolox added separately from the morula stage protected embryos against oxidative stress and improved the quality of the resulting blastocysts. |
| Databáze: | OpenAIRE |
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