Naringin Protects Against Interleukin 1β (IL-1β)-Induced Human Nucleus Pulposus Cells Degeneration via Downregulation Nuclear Factor kappa B (NF-κB) Pathway and p53 Expression
| Autor: | Chen Yu, Mingming Ji, Ting Zhang, Gang Gao, Xinhu Huang, Yufen Duan, Feng Chang, Zhaolin Hu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Nucleus Pulposus Cell Survival Interleukin-1beta Down-Regulation Inflammation Intervertebral Disc Degeneration 030204 cardiovascular system & hematology Matrix metalloproteinase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Clinical Research medicine Humans Viability assay Naringin Cells Cultured Aggrecan Cell Nucleus Interleukin-6 Tumor Necrosis Factor-alpha Chemistry NF-kappa B Interleukin General Medicine Middle Aged NFKB1 Molecular biology Matrix Metalloproteinases Cytoprotection 030220 oncology & carcinogenesis Flavanones Female Tumor necrosis factor alpha Tumor Suppressor Protein p53 medicine.symptom |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.918597 |
| Popis: | strongBACKGROUND/strongLow back pain (LBP) is regarded as a frequent disease that causes disability. We aimed to explore the effect of naringin on intervertebral disc degeneration (IDD) in IL-1ß-induced human nucleus pulposus (NP) cells and its corresponding molecular mechanisms.strongMATERIAL AND METHODS/strongHuman NP cells were identified by toluidine blue and Safranin O staining. Cell viability was determined by MTT assay. The expression levels of matrix metalloproteinases (MMP-3, MMP-13, ADAMTS-4, ADAMTS-5, collagen II, aggrecan), inflammatory genes (tumor necrosis factor [TNF]-alpha, interleukin [IL]-6), kappa B kinase alpha (IkappaBalpha), p65 and p53 were determined by quantitative real-time polymerase chain reaction (qPCR) and western blotting. Immunofluorescence study was performed to detect the position and expression of p65 protein in IL-1ß-induced human NP cells.strongRESULTS/strongHuman NP cells were successfully separated from intervertebral disc tissue. We found that naringin could significantly reduce the expressions of matrix metalloproteinases (MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5) and inflammatory genes in IL-1ß-stimulated human NP cells, while collagen II and aggrecan were increased at mRNA and protein level. Immunofluorescence showed that naringin pretreatment decreased the p65 protein expression in the nucleus and suppressed the phosphorylation of IkappaBalpha and p65.strongCONCLUSIONS/strongThese results demonstrated that naringin could attenuate matrix metalloproteinase catabolism and inflammation in IL-1ß-treated human nucleus pulposus cells via downregulating NF-kappaB pathway and p53 expression, suggesting that naringin has the potential to prevent and treat IDD. |
| Databáze: | OpenAIRE |
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