Epitope focus, clonal composition and Th1 phenotype of the human CD4 response to the secretory mycobacterial antigen Ag85
| Autor: | M. T. Valle, G. Li Pira, Luisa Lozzi, Fabrizio Manca, Annamaria Megiovanni, Andrea Merlo, Kris Huygen, Laura Bottone, Luisa Bracci, G. Angelini |
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| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cytotoxicity Immunologic T cell Immunology Epitopes T-Lymphocyte Immunodominance Biology Peripheral blood mononuclear cell Epitope Immune system Antigen medicine Immunology and Allergy Humans Interferon gamma Antigens Bacterial Cell Differentiation Immunity to Infection T lymphocyte Mycobacterium tuberculosis Th1 Cells medicine.anatomical_structure CD4 Antigens Acyltransferases medicine.drug |
| Zdroj: | Clinical and experimental immunology. 123(2) |
| ISSN: | 0009-9104 |
| Popis: | SUMMARYLymphoproliferation of healthy donors was tested against mycobacterial antigens (PPD, Ag85, Ag85 peptides). All PPD responders recognized the secretory antigen Ag85 and the peptide specificity for Ag85B was defined. Peptide 91–108 was recognized by 85% of donors. In addition, all CD4 T cell lines generated from 12 donors against PPD or Ag85 responded to 91–108. When this peptide was used to generate T cell lines, the cells responded also to tuberculins from atypical mycobacterial species. Thus the cross-reactive peptide behaved as quasi-universal. The analysis of TCR-BV gene usage by cell lines showed that most Ag85-specific T cells correspond to 91–108-specific clonotypes. Intracytoplasmic staining of cell lines after phorbol myristate acetate stimulation resulted in dominance of interferon-gamma (IFN-γ)-IL-4 double-positive cells, whereas antigen stimulation resulted in production of IFN-γ only. The data show that peptide 91–108 is the major focus of the CD4 response to mycobacterial antigens in peripheral blood mononuclear cells and in T cell lines from PPD responders. |
| Databáze: | OpenAIRE |
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