Antigen detection for the diagnosis of pneumonia
| Autor: | Maria Tereza K Campos, Altacílio Aparecido Nunes, Paulo Augusto Moreira Camargos, Petrônio Rabelo Costa |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Haemophilus Infections Urine medicine.disease_cause Gastroenterology Sensitivity and Specificity Community-acquired pneumonia Internal medicine Positive predicative value White blood cell Streptococcus pneumoniae medicine Pneumonia Bacterial Humans Child Antigens Bacterial business.industry Respiratory disease Bacterial pneumonia Haemophilus influenzae type b Pneumonia Pneumococcal medicine.disease Microspheres Community-Acquired Infections Pneumonia medicine.anatomical_structure Case-Control Studies Child Preschool Pediatrics Perinatology and Child Health Immunology Female Reagent Kits Diagnostic business Latex Fixation Tests |
| Zdroj: | Pediatric pulmonology. 38(2) |
| ISSN: | 8755-6863 |
| Popis: | Streptococcus pneumoniae and Haemophilus influenzae type b are the main agents of bacterial community-acquired pneumonia in developing countries, although a definite etiologic diagnosis cannot be established in most cases. This study was carried out to assess the performance of a latex particle agglutination test (LPAT) from a commercial kit (Slidex Meningite Kit trade mark, BioMerieux, France) in diagnosing pneumococcal and H. influenzae type b pneumonia. One hundred and seven children (45 ill subjects and 62 healthy controls) were enrolled. All 45 cases had a presumptive diagnosis of bacterial pneumonia based on clinical (WHO criteria), laboratory (white blood cell count > or = 15.000/mm3, polymorphonuclear leukocytes > or = 70%, bands > or = 500/mm3, and C-reactive protein > or = 40 mg/l), and radiological findings, i.e., two or more positive points in the scoring system described by Khamapirad and Glezen (Semin Respir Infect 1987;2:130-144). Clinical, laboratory, and radiological assessments were performed in a blinded manner. LPAT was performed in urine samples after concentration through an ethanol-acetone solution. Sensitivity, specificity, and positive and negative predictive values were 77.3% (95% CI, 61.8-88.0%), 90.3% (95% CI, 79.5-96.0%), 85.0% (95% CI, 69.5-93.8%), and 84.8% (95% CI, 73.4-92.1%), respectively. Results suggest that LPAT is a useful diagnostic tool for the etiologic diagnosis of S. pneumoniae and H. influenzae type b pneumonia, especially in the developing world. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text