High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum
| Autor: | Erik van der Vlis, Bert Ooms, Otto Halmingh, Mark van Gils, Dick van de Lagemaat, Elwin Verheij, Anniek Schellen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Sorbent Chromatography Paclitaxel Tandem Elution Chemistry Organic Chemistry Reproducibility of Results Mass spectrometry Tandem mass spectrometry Mass Spectrometry Analytical Chemistry Cartridge Evaluation Studies as Topic Humans Solid phase extraction Throughput (business) Blood Chemical Analysis Spectroscopy |
| Zdroj: | Rapid Communications in Mass Spectrometry, 14, 230-233 |
| Popis: | The feasibility of high throughput on-line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns. Copyright © 2000 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|