Comparative evaluation of PLGA nanoparticle delivery system for 5-fluorouracil and curcumin on squamous cell carcinoma
| Autor: | Mohamed H. Elmalahy, Shaimaa M. Masloub, Dina Sabry, Wael S. Mohamed, Sahar H. Ahmed |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Curcumin Apoptosis 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems 0302 clinical medicine Polylactic Acid-Polyglycolic Acid Copolymer Cell Line Tumor medicine Humans MTT assay Lactic Acid Particle Size Cytotoxicity Laryngeal Neoplasms General Dentistry Cell Proliferation Caspase 3 Squamous Cell Carcinoma of Head and Neck Chemistry Cell growth Cell Biology General Medicine Squamous carcinoma PLGA 030104 developmental biology Otorhinolaryngology Head and Neck Neoplasms 030220 oncology & carcinogenesis Cancer cell Carcinoma Squamous Cell Cancer research Nanoparticles Fluorouracil Drug Screening Assays Antitumor Polyglycolic Acid |
| Zdroj: | Archives of Oral Biology. 64:1-10 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2015.12.003 |
| Popis: | Purpose The purpose of this study is to assess the effect of 5-fluorouracil nanoparticles and curcumin naoparticles on cell proliferation and the expression of the apoptotic marker (caspase 3) in squamous cell carcinoma cell line. Material and methods PLGA 5-fluorouracil nanopartciles and PLGA curcumin nanoparticles were prepared and applied for 24 and 48 h on human laryngeal squamous carcinoma cell line (Hep-2) as regard IC 50 concentration. MTT assay was used for evaluation of cytotoxicity of prepared nanoparticles. Quantitaive reverse transcriptase polymerase chain reaction (QRT-PCR) was used for the assessment of caspase-3 expression in the treated cell line. Results The drug release rate profiles was dependent upon polymer to drug ratio, noting that the higher PLGA polymer ratio to 5-fluprouracil or curcumin drug showed faster release rates. On the other hand, the least PLGA polymer ratio to 5-fluprouracil or curcumin drug showed the slowest release rates. MTT assay revelaed that 5-fluorouracil nanoparticels or curcumin nanoparticels showed a clear cytotoxic effect on Hep-2 cell line compared to non treated cancer cells. The RT-PCR assessment of caspase-3 expression revealed that there was a significant increase in caspase-3 expression in Hep-2 cell line treated with 5-fluorouracil nanoparticles or curcumin compared to non treated cancer cells. Conclusion Curcumin nanoparticles could be more active in inducing apoptosis in short term assays (24 h) than long term assays (48 h) due to differential cellular uptake. While 5-fluorouracil nanoparticles induced higher significant apoptosis in long term (48 h) compared to curcumin group. |
| Databáze: | OpenAIRE |
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