Genomic characterization and expression analysis of four apolipoprotein A-IV paralogs in Senegalese sole (Solea senegalensis Kaup)
| Autor: | Manuel Manchado, Ana Rodríguez-Rúa, Javier Roman-Padilla, Ismael Hachero-Cruzado, Manuel Gonzalo Claros |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
animal structures Apolipoprotein B Physiology Sequence analysis Molecular Sequence Data Apolipoprotein A-IV Biology Biochemistry Synteny 03 medical and health sciences Exon Complementary DNA Sequence Homology Nucleic Acid polycyclic compounds Animals Amino Acid Sequence RNA Messenger Molecular Biology Gene Apolipoproteins A Phylogeny Genetics Paralogs fungi Intron Gene Expression Regulation Developmental A-IVGene expression Genomics Lipid transport Apolipoprotein Diet 030104 developmental biology Larva biology.protein Flatfishes lipids (amino acids peptides and proteins) Senegalese sole |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 191 |
| ISSN: | 1879-1107 |
| Popis: | The apolipoprotein A-IV (ApoA-IV) plays a key role in lipid transport and feed intake regulation. In this work, four cDNA sequences encoding ApoA-IV paralogs were identified. Sequence analysis revealed conserved structural features including the common 33-codon block and nine repeated motifs. Gene structure analysis identified four exons and three introns except for apoA-IVAa1 (with only 3 exons). Synteny analysis showed that the four paralogs were structured into two clusters (cluster A containing apoA-IVAa1 and apoA-IVAa2 and cluster B with apoA-IVBa3 and apoA-IVBa4) linked to an apolipoprotein E. Phylogenetic analysis clearly separated the paralogs according to their cluster organization as well as revealed four subclades highly conserved in Acanthopterygii. Whole-mount analyses (WISH) in early larvae (0 and 1 day post-hatch (dph)) showed that the four paralogs were mainly expressed in yolk syncytial layer surrounding the oil globules. Later, at 3 and 5 dph, the four paralogs were mainly expressed in liver and intestine although with differences in their relative abundance and temporal expression patterns. Diet supply triggered the intensity of WISH signals in the intestine of the four paralogs. Quantification of mRNA abundance by qPCR using whole larvae only detected the induction by diet at 5 dph. Moreover, transcript levels increased progressively with age except for apoA-IVAa2, which appeared as a low-expressed isoform. Expression analysis in juvenile tissues confirmed that the four paralogs were mainly expressed in liver and intestine and secondary in other tissues. The role of these ApoA-IV genes in lipid transport and the possible role of apoA-IVAa2 as a regulatory form are discussed. |
| Databáze: | OpenAIRE |
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