Cell cycle variations in chromosomal proteins of the lens epithelium
| Autor: | Norman Wainwright, Robert C. Briggs, H. Rothstein |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Mitosis
Biology Absorbance Histones Organ Culture Techniques Leucine Lens Crystalline Animals chemistry.chemical_classification DNA synthesis Tryptophan Cell Biology DNA Cell cycle Molecular biology Crystallins Amino acid Electrophoresis Histone Nucleoproteins chemistry Biochemistry biology.protein RNA Rabbits Cell Division |
| Zdroj: | Experimental cell research. 99(1) |
| ISSN: | 0014-4827 |
| Popis: | A method has been developed for the isolation and electrophoretic separation of chromosomal proteins from rabbit lens epithelium. We have analysed these proteins at a number of times after explantation of the lens into serum containing culture media. Under these conditions, the G1-arrested lens cells are induced to undergo DNA synthesis and mitosis. Qualitatively, the electrophoretic banding pattern for all the chromosomal proteins was remarkably constant throughout the times studied. The chromosomal protein labeling patterns obtained from lenses exposed to a 1 h pulse of tritiated amino acids showed increased incorporation into non-histone chromosomal proteins (NHCPs) during the latter part of the prereplicative and S phase. Histone labeling was confined to the S phase. Absorbance scanning of the stained gels reveals characteristic ratio changes between the total stainable NHCPs and histones during the cell cycle. A 62% inhibition of DNA synthesis does not seem to decrease the incorporation of [ 3 H]leucine into histones or NHCPs. |
| Databáze: | OpenAIRE |
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