Detection of cells producing anti-idiotypic antibody to thyroid stimulating hormone-reactive antibodies
| Autor: | G. N. Beall, J. Szabo, S. R. Kruger |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases medicine.drug_class Immunology Fluorescent Antibody Technique Thyrotropin Spleen Immunofluorescence Monoclonal antibody Kidney chemistry.chemical_compound Mice Thyroid-stimulating hormone Immunoglobulin Idiotypes Internal medicine medicine Animals Humans Fluorescein isothiocyanate Lung Autoantibodies Mice Inbred BALB C biology medicine.diagnostic_test Antibodies Monoclonal General Medicine Molecular biology medicine.anatomical_structure Endocrinology chemistry Liver Microscopy Fluorescence Biotinylation Monoclonal biology.protein Antibody |
| Zdroj: | Immunological investigations. 18(7) |
| ISSN: | 0882-0139 |
| Popis: | BALB/c mice were immunized either with bovine (b) or human (h) thyroid stimulating hormone (TSH) or with one of several mouse monoclonal anti-hTSH antibodies. The binding of biotinylated mouse monoclonal anti-hTSH to sections of spleen, kidney, liver and lung was assessed with fluorescein isothiocyanate labelled avidin. Clusters of cells in the spleens of mice immunized with bTSH bound the labelled monoclonal anti-TSH. We suggest that these cells synthesized auto-anti-idiotypic antibodies to anti-TSH. |
| Databáze: | OpenAIRE |
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