No influence of OPG and its ligands, RANKL and TRAIL, on proliferation and regulation of the calcification process in primary human vascular smooth muscle cells
| Autor: | Birgitte Hviid Mumm, Vibe Skov, Mie Mechta, Lars Melholt Rasmussen, Malene Olesen |
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| Rok vydání: | 2012 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Vascular smooth muscle medicine.medical_treatment Myocytes Smooth Muscle Biochemistry Muscle Smooth Vascular TNF-Related Apoptosis-Inducing Ligand Endocrinology Osteoprotegerin Internal medicine medicine Humans Insulin Myocyte Vascular Calcification Molecular Biology Cells Cultured Cell Proliferation Oligonucleotide Array Sequence Analysis Gene knockdown biology Cell growth Chemistry RANK Ligand medicine.disease RANKL Gene Knockdown Techniques Cancer research biology.protein RNA Interference Transcriptome Calcification |
| Zdroj: | Olesen, M, Skov, V, Mechta, M, Mumm, B H & Rasmussen, L M 2012, ' No influence of OPG and its ligands, RANKL and TRAIL, on proliferation and regulation of the calcification process in primary human vascular smooth muscle cells ', Molecular and Cellular Endocrinology, vol. 362, no. 1-2, pp. 149-56 . https://doi.org/10.1016/j.mce.2012.06.004 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2012.06.004 |
| Popis: | The aim of this study was to examine the effects of the OPG-RANKL-TRAIL system on proliferation, regulation of calcification-associated genes and calcification of human vascular smooth muscle cells (HVSMCs). Small interfering (si)RNA-mediated knockdown of OPG was followed by treatment of HVSMCs with recombinant RANKL or TRAIL. Regulation of a calcification-associated gene set was assayed by pathway analysis of microarray results. The lack of OPG in HVSMCs or treatment with RANKL or TRAIL did not affect proliferation of HVSMCs. In addition, OPG, RANKL or TRAIL did not modify the regulation of a calcification-associated gene set. Finally, in the long term calcification assay, we found that cells isolated from seven different human donors showed a great variability in the response to RANKL and insulin. However, overall RANKL and/or insulin did not affect the development of calcification of HVSMCs. These studies indicate that OPG knockdown does not alter the calcification process in HVSMCs. |
| Databáze: | OpenAIRE |
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