The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum
| Autor: | Tianyong Zheng, Adam M. Guss, Sean Jean-Loup Murphy, Daniel G. Olson, Lee R. Lynd, Shuen Hon, Marybeth Maloney, Anthony A. Lanahan, Beth Papanek, Evert K. Holwerda |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ethanol biology 030106 microbiology Bioengineering biology.organism_classification Applied Microbiology and Biotechnology carbohydrates (lipids) Clostridium thermocellum 03 medical and health sciences chemistry.chemical_compound chemistry Biochemistry Bacterial Proteins Cellulosic ethanol Yield (chemistry) Hemicellulose Ethanol fuel Ethanol metabolism Cellulose Thermoanaerobacterium Biotechnology |
| Zdroj: | Metabolic engineering. 42 |
| ISSN: | 1096-7184 |
| Popis: | Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity. The four genes alone, however, were insufficient to achieve in C. thermocellum the ethanol yields and titers observed in engineered T. saccharolyticum strains, even when combined with gene deletions targeting hydrogen production. This suggests that other parts of T. saccharolyticum metabolism may also be necessary to reproduce the high ethanol yield and titer phenotype in C. thermocellum. |
| Databáze: | OpenAIRE |
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