Primary dissociated cell culture of embryonic rat metencephalon: presence of GABA in serotonergic neurons
| Autor: | Michelle Fèvre-Montange, M. Didier-Bazes, Marie-Françoise Belin, M. Ehret, Marcienne Tardy, Michel Maitre, Ariel Reboul |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Serotonin
Glutamate decarboxylase Immunocytochemistry Biology Tritium Serotonergic Pons medicine Animals Microscopy Phase-Contrast Cells Cultured gamma-Aminobutyric Acid Neurons General Neuroscience Biological Transport Tryptophan hydroxylase Embryo Mammalian Immunohistochemistry Rats medicine.anatomical_structure nervous system Autoradiography GABAergic Neuron Neuroscience Metencephalon |
| Zdroj: | Neuroscience Letters. 125:101-106 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(91)90001-a |
| Popis: | This study was performed to determine whether neurons, where γ-aminobutyric acid (GABA) and serotonin (5-HT) coexist, represent neuronal entities which can survive in vitro. In dissociated cultures from 18-day-old embryonic rat metencephalon, it was possible to develop glial and neuronal cells. Among the neurons, some of them, which contain glutamate decarboxylase or are capable of accumulating [ 3 H]GABA are GABAergic; others, containing tryptophan hydroxylase or 5-HT are serotoninergic. By combining radioautography and immunocytochemistry, it was possible to observe neurons where 5-HT and GABA coexist. Cultures might be a suitable model to study the functioning (release or synthesis of both neurotransmitters) of neurons where two classical neurotransmitters coexist. |
| Databáze: | OpenAIRE |
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