11β-hydroxysteroid dehydrogenases-2 decreases the apoptosis of MC3T3/MLO-Y4 cells induced by glucocorticoids
| Autor: | Fuling Zhou, Yaoqing Wang, Hao Zhang, Xiangpeng Bu, Fan Chen, Zhenyu Pan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system medicine.medical_treatment Biophysics Apoptosis Biology Osteocytes Biochemistry Dexamethasone Cell Line Steroid Mice 03 medical and health sciences Plasmid medicine Animals fas Receptor MC3T3 RNA Small Interfering Glucocorticoids Molecular Biology Caspase 8 Osteoblasts Steroid induced osteonecrosis Cell Differentiation Cell Biology Hydroxysteroid Dehydrogenases Cell biology 030104 developmental biology Gene Expression Regulation Proto-Oncogene Proteins c-bcl-2 UVB-induced apoptosis 11-beta-Hydroxysteroid Dehydrogenases Signal Transduction medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 490:1399-1406 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2017.07.046 |
| Popis: | The aim of the present study was to confirm the role of 11β-hydroxysteroid dehydrogenases type 2(11β-HSD-2) in steroid induced osteonecrosis of the femoral head(SANFH). We cultured mouse bone-like cells (MLO-Y4) and mouse osteoblast-like cells (MC3T3-E1). After overexpressed 11β-HSD-2 successfully, we induced cell apoptosis by dexamethasone (DXM). The level of cell apoptosis, the expression of Bcl-2 in MLO-Y4 cells and the expression of Fas and caspase8 in MC3T3-E1 cells were detected. Then, we constructed 11β-HSD-2 siRNA plasmid and represented it on MLO-Y4/MC3T3-E1 Cells, to down-regulate the 11β-HSD-2 expression. After that, we used dexamethasone to induce cell apoptosis. The level of cell apoptosis, the expression of Bcl-2 in MLO-Y4 cells and the expression of Fas and caspase8 in MC3T3-E1 cells were detected again. In the overexpression model of cells, we found that the amount of cell apoptosis, the expression of Fas and caspase8 in MC3T3-E1 cells are lower than that of control groups. The amount of cell apoptosis, the expression of Fas and caspase8 in MC3T3-E1 cells were more than before when we reduced the expression of 11β-HSD-2. In our study, we concluded that 11β-HSD-2 plays an important role in the development of bone or osteoblast cell apoptosis, and the decreased expression of 11β-HSD-2 may aggravate steroid induced bone/osteoblast cell apoptosis. |
| Databáze: | OpenAIRE |
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