Impedance-Based Monitoring of Mesenchymal Stromal Cell Three-Dimensional Proliferation Using Aerosol Jet Printed Sensors: A Tissue Engineering Application
| Autor: | Luciana Sartore, Federica Re, Kamol Dey, Nicola Lopomo, Mauro Serpelloni, Giovanna Piovani, Emilio Sardini, Michele Guindani, Andrea Bianchetti, Simona Braga, Edoardo Cantu, Camillo Almici, Mirella Marini, Sarah Tonello, Domenico Russo |
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| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Stromal cell Materials science Bioengineering 02 engineering and technology lcsh:Technology Capacitance Article aerosol jet printing 03 medical and health sciences Engineering Tissue engineering General Materials Science lcsh:Microscopy Electrical impedance lcsh:QC120-168.85 030304 developmental biology 0303 health sciences lcsh:QH201-278.5 lcsh:T Mesenchymal stem cell Phase angle 021001 nanoscience & nanotechnology 3D monitoring lcsh:TA1-2040 impedance-based cell spectroscopy tissue engineering Aerosol jet printing Impedance-based cell spectroscopy Mesenchymal stromal cells Chemical Sciences lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) mesenchymal stromal cells 0210 nano-technology lcsh:TK1-9971 Polyimide Biotechnology Biomedical engineering |
| Zdroj: | Materials (Basel, Switzerland), vol 13, iss 10 Materials Volume 13 Issue 10 Materials, Vol 13, Iss 2231, p 2231 (2020) |
| ISSN: | 1996-1944 |
| Popis: | One of the main hurdles to improving scaffolds for regenerative medicine is the development of non-invasive methods to monitor cell proliferation within three-dimensional environments. Recently, an electrical impedance-based approach has been identified as promising for three-dimensional proliferation assays. A low-cost impedance-based solution, easily integrable with multi-well plates, is here presented. Sensors were developed using biocompatible carbon-based ink on foldable polyimide substrates by means of a novel aerosol jet printing technique. The setup was tested to monitor the proliferation of human mesenchymal stromal cells into previously validated gelatin-chitosan hybrid hydrogel scaffolds. Reliability of the methodology was assessed comparing variations of the electrical impedance parameters with the outcomes of enzymatic proliferation assay. Results obtained showed a magnitude increase and a phase angle decrease at 4 kHz (maximum of 2.5 kΩ and &minus 9 degrees) and an exponential increase of the modeled resistance and capacitance components due to the cell proliferation (maximum of 1.5 kΩ and 200 nF). A statistically significant relationship with enzymatic assay outcomes could be detected for both phase angle and electric model parameters. Overall, these findings support the potentiality of this non-invasive approach for continuous monitoring of scaffold-based cultures, being also promising in the perspective of optimizing the scaffold-culture system. |
| Databáze: | OpenAIRE |
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