Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China
| Autor: | Mengyan Zhang, Guo-jun Hu, Zunping Zhang, Yafeng Dong, Xudong Fan, Fang Ren |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Agriculture (General) polyprotein gene RT-PCR Plant Science Biology 01 natural sciences Biochemistry S1-972 Food Animals GFabV Cultivar Gene Genetics Genetic diversity Ecology Phylogenetic tree phylogenetic analysis food and beverages 04 agricultural and veterinary sciences Grapevine fabavirus 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 19, Iss 3, Pp 768-774 (2020) |
| ISSN: | 2095-3119 |
| DOI: | 10.1016/s2095-3119(19)62677-8 |
| Popis: | A total of 288 grapevine samples of 61 different grapevine cultivars, collected from 22 provinces and regions, were analyzed by reverse transcription-PCR (RT-PCR) for the presence of grapevine fabavirus (GFabV). PCR detection results showed the incidences of GFabV were 12.8% (30/235) and 48.1% (25/52) in the asymptomatic and symptomatic vines, respectively. The genetic diversity of GFabV isolates was analyzed based on partial nucleotide and encoded amino acid sequences of the RNA1 and RNA2 polyprotein genes. Phylogenetic analyses of the RNA1 and RNA2 gene sequences divided the GFabV isolates into five well-defined groups. Groups 1, 2, and 4 comprised only Chinese isolates. This article represents the first report for the prevalence and genetic diversity of GFabV in grapevines grown in China. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect