DNA flow cytometry of control Euglena and cell cycle blockade of vitamin B12-starved cells
| Autor: | M. H. Bre, P. Manigault, M. Lefort-Tran, M. Pouphile |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
DNA Replication
Euglena gracilis ved/biology.organism_classification_rank.species Biophysics Euglena Pathology and Forensic Medicine Flow cytometry Endocrinology medicine Mitosis Interphase medicine.diagnostic_test DNA synthesis biology ved/biology DNA replication Cell Biology Hematology DNA Cell cycle biology.organism_classification Flow Cytometry Cell biology Microscopy Electron Vitamin B 12 Biochemistry Cell culture Bisbenzimidazole |
| Zdroj: | Cytometry. 8(1) |
| ISSN: | 0196-4763 |
| Popis: | Vitamin B12 starvation in Euglena induces a cell cycle arrest that leads to unbalanced growth. Microfluorometry and flow cytometry analyses of cellular DNA fluorescence after Hoechst 33258 staining were performed on control and vitamin B12-deficient cells. Convergent results are obtained with both methods. Histograms that represent arrested cells are unimodal, with a mode channel value nearly twice that of the G1 control cell peak. Dispersion of fluorescence values is great, and values from 2C and over 4C are observed and discussed. It appears that vitamin B12 starvation in Euglena leads to defective DNA synthesis. Blocked cells have different DNA content, corresponding to blockade of DNA replication during the S phase. A second block prevents the onset of mitosis even for 4C cells. |
| Databáze: | OpenAIRE |
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