Differential Cytotoxic Potential of Acridocarpus orientalis Leaf and Stem Extracts with the Ability to Induce Multiple Cell Death Pathways

Autor: Mohammed M. Qureshi, Farah Mustafa, Waqar Ahmad, Najeeb Ur Rehman, Javid Hussain, Liaqat Ali, Shaima Akhlaq, Ahmed Al-Harrasi, Sameera Omar Mohammed Saeed Balhamar, Neena G. Panicker
Rok vydání: 2019
Předmět:
pharmacognosy
Uterine Cervical Neoplasms
Pharmaceutical Science
Analytical Chemistry
HeLa
0302 clinical medicine
Drug Discovery
Cytotoxic T cell
Cytotoxicity
0303 health sciences
Plant Stems
biology
Chemistry
traditional herbal medicine
apoptosis
Cell killing
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
MCF-7 Cells
Molecular Medicine
Female
molecular mechanism
acridocarpus orientalis
Malpighiaceae
autophagy
Programmed cell death
Necroptosis
necroptosis
Breast Neoplasms
anticancer
Article
lcsh:QD241-441
Acridocarpus orientalis
03 medical and health sciences
lcsh:Organic chemistry
Humans
Physical and Theoretical Chemistry
Cell Proliferation
030304 developmental biology
Plant Extracts
Organic Chemistry
phytotherapy
phytochemicals
biology.organism_classification
Antineoplastic Agents
Phytogenic
Molecular biology
Plant Leaves
Apoptosis
Cancer cell
HeLa Cells
Zdroj: Molecules, Vol 24, Iss 21, p 3976 (2019)
Molecules
Volume 24
Issue 21
ISSN: 1420-3049
DOI: 10.3390/molecules24213976
Popis: This study systematically analyzed the anticancer potential of Acridocarpus orientalis (AO), a traditional medicinal plant of the Arabian Peninsula/East Africa known for its anti-inflammatory and pain relief properties. Tests of serial organic fractions from methanolic extracts of its leaves and stems revealed that only some fractions showed anti-proliferative potential with the dichloromethane fraction from leaves (AOD (L)) showing the most cytotoxic effect against both breast (MCF-7 and MDA-MB-231) and cervical (HeLa) cancer cell lines. The n-butanol fraction from the stems (AOB (S)), on the other hand, was more effective against cervical cancer cells and did not harm the normal cells. Further characterization of the mode of cell killing revealed that AOD (L) depended more on non-apoptotic pathways for its cytotoxicity in breast cancer cells, while it could activate some apoptosis and necroptosis in HeLa cells. The AOB (S) fraction could primarily activate apoptosis and some necroptosis in HeLa cells. Both fractions perturbed autophagy, but in a dissimilar manner. Thus, different parts of A. orientalis revealed variable potential to induce cell death in cancer cells via apoptotic and non-apoptotic pathways, making A. orientalis a valuable plant for the exploration of anticancer bioactive reagents, some of which may be protective for normal cells.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje