Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats
| Autor: | Guilherme R Motta, Isabel Rodrigues Rosado, Fabíola Bono Fukushima, Anna Luiza Fv Assumpção, Saira M. N Neves, Mário Sérgio Lima de Lavor, Karen Maciel de Oliveira, Eliane Gonçalves de Melo, C.M. Silva, Marcus Vinicius Gomez, Fernanda F Garcia, Marília Martins Melo |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Conus magus Histopathology Spleen Pharmacology Placebo Toxicology Neuroprotection Biochemistry lcsh:RA1190-1270 lcsh:Zoology medicine lcsh:QL1-991 Adverse effect Spinal cord injury lcsh:Toxicology. Poisons Kidney business.industry Stomach Research Hematology medicine.disease Cone snail medicine.anatomical_structure Infectious Diseases Animal Science and Zoology Parasitology business Marine toxin |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0 (2014) Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 20, Article number: 15, Published: 09 MAY 2014 The Journal of Venomous Animals and Toxins Including Tropical Diseases Journal of Venomous Animals and Toxins including Tropical Diseases v.20 2014 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1678-9199 |
| Popis: | Background: Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitro models of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes. Results: The clinical, hematologic and biochemical evaluation revealed no significant abnormalities in all groups, even in high doses. There was no significant alteration in organs, except for degenerative changes in kidneys at a dose of 120 pmol. Conclusions: These findings suggest that MVIIC at 15, 30 and 60 pmol are safe for intralesional administration after spinal cord injury and could be further investigated in relation to its neuroprotective effects. However, 120 pmol doses of MVIIC may provoke adverse effects on kidney tissue. |
| Databáze: | OpenAIRE |
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