Vasculin, a novel vascular protein differentially expressed in human atherogenesis
| Autor: | Sylvia Heeneman, Peter J. E. H. M. Kitslaar, Ann Gils, Ann P. J. J. Bijnens, Abraham A. Kroon, Barry Jutten, Kitty B. J. M. Cleutjens, Birgit C. G. Faber, Mat J.A.P. Daemen, Jan H. M. Tordoir, Carlie J.M. de Vries |
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| Přispěvatelé: | Amsterdam Cardiovascular Sciences, Medical Biochemistry, Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
DNA
Complementary Arteriosclerosis Immunology Blotting Western Molecular Sequence Data Biology Biochemistry Schistosoma japonicum Cell Line Exon Western blot Peptide Library Complementary DNA medicine Animals Humans Electrophoresis Gel Two-Dimensional Tissue Distribution Northern blot Amino Acid Sequence RNA Messenger Immunoglobulin Fragments Cells Cultured Gene Library Glutathione Transferase Messenger RNA medicine.diagnostic_test Base Sequence cDNA library Alternative splicing Cell Biology Hematology Blood Proteins Exons Blotting Northern Molecular biology Immunohistochemistry Introns Recombinant Proteins Protein Structure Tertiary DNA-Binding Proteins Alternative Splicing Suppression subtractive hybridization Plasmids |
| Zdroj: | Blood, 102(8), 2803-2810. American Society of Hematology |
| ISSN: | 0006-4971 |
| DOI: | 10.1182/blood-2003-01-0306 |
| Popis: | Recent suppressive subtractive hybridization analysis on human atherosclerotic plaque-derived RNA revealed genes upregulated in plaques with a thrombus versus stable plaques. Clone SSH6, containing part of a putative open reading frame of an unknown protein, was further investigated. Full-length cDNA, coding for a 473–amino acid (aa) protein, was identified in a vascular smooth muscle cell (SMC) cDNA library. Bioinformatics suggested the presence of multiple SSH6 variants due to alternative splicing of exon 3. Multiple-tissue Northern blot analysis demonstrated a differential expression pattern of these variants, as a ubiquitously expressed SSH6 mRNA missing exon 3, was detected apart from a putative vascular SMC–specific form containing exon 3. Western blot analysis indicated a ubiquitous 35-kDa protein (SSH6-β), in addition to a 45-kDa protein (vasculin), detected in the vascular wall and in plasma. Analysis of arteries displaying various stages of atherosclerosis indicated that the vasculin/SSH6-β ratio increases throughout atherogenesis. Immunohistochemical analysis demonstrated cytoplasmic expression of SSH6 gene products in macrophages, endothelial cells, and SMCs. In summary, we identified a novel mRNA/protein, vasculin, in the arterial wall and plasma. The regulated expression of vasculin in plaques suggests a role in atherogenesis. Moreover, its presence in plasma opens perspectives for vasculin as a marker for atherosclerosis. |
| Databáze: | OpenAIRE |
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