High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays
| Autor: | Saeid Ansaryan, Yen-Cheng Liu, Xiaokang Li, Augoustina Maria Economou, Christiane Sigrid Eberhardt, Camilla Jandus, Hatice Altug |
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| Popis: | Methods for the analysis of cell secretions at the single-cell level only provide semiquantitative endpoint readouts. Here we describe a microwell array for the real-time spatiotemporal monitoring of extracellular secretions from hundreds of single cells in parallel. The microwell array incorporates a gold substrate with arrays of nanometric holes functionalized with receptors for a specific analyte, and is illuminated with light spectrally overlapping with the device's spectrum of extraordinary optical transmission. Spectral shifts in surface plasmon resonance resulting from analyte-receptor bindings around a secreting cell are recorded by a camera as variations in the intensity of the transmitted light while machine-learning-assisted cell tracking eliminates the influence of cell movements. We used the microwell array to characterize the antibody-secretion profiles of hybridoma cells and of a rare subset of antibody-secreting cells sorted from human donor peripheral blood mononuclear cells. High-throughput measurements of spatiotemporal secretory profiles at the single-cell level will aid the study of the physiological mechanisms governing protein secretion. A plasmonic microwell array incorporating a gold substrate with arrays of receptor-coated nanometric holes enables the imaging-based spatiotemporal monitoring of secreted proteins from hundreds of single cells at sub-minute resolution. |
| Databáze: | OpenAIRE |
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