Detection of Chlamydia pneumoniae but not cytomegalovirus in occluded saphenous vein coronary artery bypass grafts
| Autor: | Claus Bartels, F. Sayk, A. C. Feller, Matthias Maass, J. F. M. Bechtel, R. Malisius, N. Brill, Hans-H. Sievers, Gregor Bein |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
DNA Bacterial Male Pathology medicine.medical_specialty Congenital cytomegalovirus infection Cytomegalovirus Pathogenesis Physiology (medical) medicine Humans Chlamydiaceae Saphenous Vein Coronary Artery Bypass Vein Aged biology Vascular disease business.industry Graft Occlusion Vascular Chlamydophila pneumoniae Middle Aged medicine.disease biology.organism_classification medicine.anatomical_structure Real-time polymerase chain reaction DNA Viral Female Cardiology and Cardiovascular Medicine business Nested polymerase chain reaction Artery |
| Zdroj: | Circulation. 99(7) |
| ISSN: | 0009-7322 |
| Popis: | Background —A causal relation between atherosclerosis and chronic infection with Chlamydia pneumoniae and/or cytomegalovirus (CMV) has been suggested. Whether the unresolved problem of venous coronary artery bypass graft occlusion is related to infection with C pneumoniae and/or CMV has not been addressed. Methods and Results —Thirty-eight occluded coronary artery vein grafts and 20 native saphenous veins were examined. Detection of C pneumoniae DNA was performed by use of nested polymerase chain reaction (PCR). Homogenisates from the specimen were cultured for identification of viable C pneumoniae . Both conventional PCR and quantitative PCR for detection of CMV DNA were applied. Differential pathological changes (degree of inflammation, smooth muscle cell proliferation [MIB-1]) were determined and correlated to the detection of both microorganisms. C pneumoniae DNA could be detected in 25% of occluded vein grafts. Viable C pneumoniae was recovered from 16% of occluded vein grafts. Except for 1 native saphenous vein, all control vessels were negative for both C pneumoniae detection and culture. All pathological and control specimens were negative for CMV DNA detection. Pathological changes did not correlate with C pneumoniae detection. Conclusions —Occluded aorto-coronary venous grafts harbor C pneumoniae but not CMV. The detection of C pneumoniae in occluded vein grafts warrants further investigation. |
| Databáze: | OpenAIRE |
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