Structure of the Mucosal and Stool Microbiome in Lynch Syndrome
| Autor: | Melanie P. Parziale, Yan Yan, Zsofia K. Stadler, Peter B. Kelsey, Zalak Patel, Galeb Abu-Ali, David A. Drew, James M. Richter, Christina Tran, Arnold J. Markowitz, Katherine K. Gilpin, Curtis Huttenhower, Madeline M. Schuck, Long H. Nguyen, Jason Lloyd-Price, Dana Meixell, Daniel C. Chung, Wendy S. Garrett, Andrew T. Chan |
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| Rok vydání: | 2020 |
| Předmět: |
Adenoma
Adult Male medicine.medical_specialty Colorectal cancer medicine.medical_treatment Gut flora Microbiology Gastroenterology Article Feces 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S Virology Internal medicine Tumor Microenvironment medicine Humans Clostridiaceae Prospective Studies Microbiome Colectomy Aged 030304 developmental biology Aged 80 and over 0303 health sciences biology Human microbiome Middle Aged biology.organism_classification medicine.disease Colorectal Neoplasms Hereditary Nonpolyposis digestive system diseases Lynch syndrome Gastrointestinal Microbiome Metagenomics Colonic Neoplasms Female Parasitology Transcriptome 030217 neurology & neurosurgery |
| Zdroj: | Cell Host Microbe |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2020.03.005 |
| Popis: | The gut microbiota has been associated with colorectal cancer (CRC), but causal alterations preceding CRC have not been elucidated. To prospectively assess microbiome changes prior to colorectal neoplasia, we investigated samples from 100 Lynch syndrome patients using 16S rRNA gene sequencing of colon biopsies, coupled with metagenomic and metatranscriptomic sequencing of feces. Colectomy and CRC history represented the largest effects on microbiome profiles. A subset of Clostridiaceae were depleted in stool corresponding with baseline adenomas, while Desulfovibrio was enriched both in stool and in mucosal biopsies. A classifier leveraging stool metatranscriptomes resulted in modest power to predict interval development of preneoplastic colonic adenoma. Predictive transcripts corresponded with a shift in flagellin contributors and oxidative metabolic microenvironment, potentially factors in local CRC pathogenesis. This suggests that the effectiveness of prospective microbiome monitoring for adenomas may be limited, but supports the potential causality of these consistent, early microbial changes in colonic neoplasia. |
| Databáze: | OpenAIRE |
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