Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors
| Autor: | Seema S Gangolli, Nikos Vasilakis, Darlene Falvey, Timothy J. Zamb, Stephen A. Udem, Jacek Kowalski, Gerald R. Kovacs, John W. Coleman |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Gene Expression Regulation
Viral animal structures viruses Genetic Vectors Biomedical Engineering Vaccinia virus Bioengineering Alphavirus Transfection Recombinant virus complex mixtures Applied Microbiology and Biotechnology Microbiology Encephalitis Virus Venezuelan Equine chemistry.chemical_compound Drug Delivery Systems Virus-like particle Replicon Orthopoxvirus Vector (molecular biology) biology Gene Transfer Techniques Virion virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification Virology chemistry DNA Viral Togaviridae Molecular Medicine Vaccinia Biotechnology |
| Zdroj: | Nature Biotechnology. 21:932-935 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/nbt845 |
| Popis: | This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (VEE) virus replicon particles (VRP). Infection of mammalian cells with these recombinant MVA vectors resulted in robust expression of VEE structural genes, replication of the alphavirus vector and high titers of VRP. In addition, VRP packaging was achieved in a cell type (fetal rhesus lung) that has been approved for the manufacturing of vaccines destined for human use. |
| Databáze: | OpenAIRE |
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