Monooxygenase-Mediated 1,2-Dichloroethane Degradation by Pseudomonas sp. Strain DCA1
| Autor: | Jacobus C. Hage, Sybe Hartmans |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Ecology
Strain (chemistry) Stereochemistry Chloroacetic acid Monooxygenase Applied Microbiology and Biotechnology Redox Culture Media chemistry.chemical_compound Hydrolysis Biodegradation Environmental Environmental and Public Health Microbiology chemistry Biofilms Pseudomonas Oxygenases Chloroacetaldehyde Organic chemistry Ethylene Dichlorides Energy source Oxidation-Reduction Glycolic acid Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 65:2466-2470 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.65.6.2466-2470.1999 |
| Popis: | A bacterial strain, designated Pseudomonas sp. strain DCA1, was isolated from a 1,2-dichloroethane (DCA)-degrading biofilm. Strain DCA1 utilizes DCA as the sole carbon and energy source and does not require additional organic nutrients, such as vitamins, for optimal growth. The affinity of strain DCA1 for DCA is very high, with a K m value below the detection limit of 0.5 μM. Instead of a hydrolytic dehalogenation, as in other DCA utilizers, the first step in DCA degradation in strain DCA1 is an oxidation reaction. Oxygen and NAD(P)H are required for this initial step. Propene was converted to 1,2-epoxypropane by DCA-grown cells and competitively inhibited DCA degradation. We concluded that a monooxygenase is responsible for the first step in DCA degradation in strain DCA1. Oxidation of DCA probably results in the formation of the unstable intermediate 1,2-dichloroethanol, which spontaneously releases chloride, yielding chloroacetaldehyde. The DCA degradation pathway in strain DCA1 proceeds from chloroacetaldehyde via chloroacetic acid and presumably glycolic acid, which is similar to degradation routes observed in other DCA-utilizing bacteria. |
| Databáze: | OpenAIRE |
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