Effect of lipopeptide structure on gene delivery system properties: Evaluation in 2D and 3D in vitro models
| Autor: | M.G. Drozdova, Elena Markvicheva, Dmitry Kudlay, Alexander M. Sapozhnikov, I P Shilovskiy, Yu. L. Sebyakin, Anastasia Gileva, M.V. Grechihina, Elena I. Kovalenko, Musa Khaitov, N.E. Suzina, O. O. Koloskova, U. A. Budanova |
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| Rok vydání: | 2018 |
| Předmět: |
Cell Survival
Surface Properties 02 engineering and technology Gene delivery Transfection 010402 general chemistry Models Biological 01 natural sciences Lipopeptides Colloid and Surface Chemistry Amphiphile Humans Cationic liposome Particle Size RNA Small Interfering Physical and Theoretical Chemistry Cells Cultured Cell Proliferation chemistry.chemical_classification Liposome Microscopy Confocal Chemistry Gene Transfer Techniques Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Amino acid HEK293 Cells Liposomes Biophysics Nucleic acid 0210 nano-technology HeLa Cells Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 167:328-336 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2018.04.003 |
| Popis: | Development of efficient biodegradable, environmentally responsive, biocompatible and non-toxic delivery system is needed for efficient gene delivery. As well known, properties of the vehicle are determined by the structure of carrier components. The aim of the current study was to estimate in vitro transfection efficacy of aliphatic di-, tri- and tetrapeptide-based cationic lipoplexes loaded with siRNA in function of a number of cationic groups using 2D (monolayer culture) and 3D (multicellular tumor spheroids) in vitro models. Physicochemical properties and cytotoxicity of the liposomes were found to be dependent upon a number of amino acid derivatives in an amphiphilic polar head. Uptake of liposomes loaded with nucleic acid (lipoplexes) and their localization in HEK293T cells was studied by confocal microscopy. The liposomes based on lipotripeptides had the highest transfection efficiency which was 20-fold higher than those fabricated from lipotetrapeptides. |
| Databáze: | OpenAIRE |
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