Oil-encapsulating PEO-PPO-PEO/PEG shell cross-linked nanocapsules for target-specific delivery of paclitaxel
| Autor: | Kh Bae Ki-Hyun Bae, Tg Park Tae Gwan Park, Yuhan Lee |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Polymers and Plastics
Paclitaxel Sonication Bioengineering Apoptosis Receptors Cell Surface macromolecular substances Adenocarcinoma Nanocapsules Polyethylene Glycols Biomaterials chemistry.chemical_compound Drug Delivery Systems Folic Acid Dynamic light scattering Cell Line Tumor PEG ratio Polymer chemistry Materials Chemistry Humans Chemistry Folate Receptors GPI-Anchored technology industry and agriculture Iodized Oil Cross-Linking Reagents Chemical engineering Solubility Folate receptor Propylene Glycols Drug delivery Drug carrier Carrier Proteins Ethylene glycol Oils |
| Zdroj: | Biomacromolecules. 8(2) |
| ISSN: | 1525-7797 |
| Popis: | PEO-PPO-PEO/PEG shell cross-linked nanocapsules encapsulating an oil phase in their nanoreservoir structure was developed as a target-specific carrier for a water-insoluble drug, paclitaxel. Oil-encapsulating PEO-PPO-PEO/PEG composite nanocapsules were synthesized by dissolving an oil (Lipiodol) and an amine-reactive PEO-PPO-PEO derivative in dichloromethane and subsequently dispersing in an aqueous solution containing amine-functionalized six-arm-branched poly(ethylene glycol) by ultrasonication. The resultant shell cross-linked nanocapsules had a unique core/shell architecture with an average size of 110.7 +/- 9.9 nm at 37 degrees C, as determined by dynamic light scattering and transmission electron microscopy. Paclitaxel could be effectively solubilized in the inner Lipiodol phase surrounded by a cross-linked PEO-PPO-PEO/PEG shell layer. The paclitaxel-loaded nanocapsules were further conjugated with folic acid to achieve folate receptor targeted delivery. Confocal microscopy and flow cytometric analysis revealed that folate-mediated targeting significantly enhanced the cellular uptake and apoptotic effect against folate receptor overexpressing cancer cells. The present study suggested that these novel nanomaterials encapsulating an oil reservoir could be potentially applied for cancer cell targeted delivery of various water-insoluble therapeutic and diagnostic agents. |
| Databáze: | OpenAIRE |
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