Multimodal molecular imaging of atherosclerosis: Nanoparticles functionalized with scFv fragments of an anti-αIIbβ3 antibody

Autor: Stéphane Mornet, Jeanny Laroche-Traineau, Carmelo Di Primo, Laetitia Minder, Xavier Santarelli, Gisèle Clofent-Sanchez, Mélusine Larivière, Stéphane Sanchez, Samuel Bonnet, Cyril Lorenzato, Laurent Adumeau, Audrey Hemadou, Marie-Josée Jacobin-Valat, Abdelmajid Noubhani
Přispěvatelé: Centre de résonance magnétique des systèmes biologiques (CRMSB), Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut Bergonié [Bordeaux], UNICANCER, Régulations Naturelles et Artificielles (ARNA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux Ségalen [Bordeaux 2], Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Européen de Chimie et de Biologie, Bordeaux - Recherche Intégrée en Oncologie (SIRIC-BRIO), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This work has benefited from the Vivoptic imaging platform supported by France Life Imaging. More particularly, we would like to thank C. Genevois for her technical support. This work has benefited from the facilities and expertise of the Biophysical and Structural Chemistry plateform (BPCS) at IECB, CNRS UMS3033, Inserm US001, Bordeaux University http://www.iecb.u-bordeaux.fr/index.php/fr/plateformestechnologiques. The microscopy was done in the Bordeaux Imaging Center a service unit of the CNRS-INSERM and Bordeaux University, member of the national infrastructure France BioImaging supported by the French National Research Agency (ANR-10-INBS-04). The help of Sébastien Marais is acknowledged. We would like to thank the Haut-Lévèque Hospital in Pessac, France, for providing the human biological material., ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), Admin, Oskar
Rok vydání: 2018
Předmět:
Male
Integrin
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Near infrared fluorescence (NIRF)
Bioengineering
02 engineering and technology
Platelet Glycoprotein GPIIb-IIIa Complex
Multimodal Imaging
Fluorescence
03 medical and health sciences
Mice
In vivo
medicine
Bimodal imaging
Animals
General Materials Science
Avidity
Tissue Distribution
Platelet activation
030304 developmental biology
[CHIM.MATE] Chemical Sciences/Material chemistry
0303 health sciences
biology
Chemistry
Directed conjugation
[CHIM.MATE]Chemical Sciences/Material chemistry
021001 nanoscience & nanotechnology
medicine.disease
Atherosclerosis
scFv antibody fragments
Magnetic Resonance Imaging
3. Good health
Molecular Imaging
Atheroma
SPIO
biology.protein
Biophysics
Molecular Medicine
Nanoparticles
Rabbits
Antibody
Molecular imaging
0210 nano-technology
Ex vivo
Single-Chain Antibodies
Zdroj: Nanomedicine
Nanomedicine, Future Medicine, 2019, 22, pp.102082-102082
Nanomedicine: Nanotechnology, Biology and Medicine
Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2019, 22, 102082 (12 p.). ⟨10.1016/j.nano.2019.102082⟩
ISSN: 1549-9642
1743-5889
1748-6963
1549-9634
DOI: 10.1016/j.nano.2019.102082⟩
Popis: International audience; Due to the wealth of actors involved in the development of atherosclerosis, molecular imaging based on the targeting of specific markers would substantiate the diagnosis of life-threatening atheroma plaques. To this end, TEG4 antibody is a promising candidate targeting the activated platelets (integrin αIIbβ3) highly represented within the plaque. In this study, scFv antibody fragments were used to functionalize multimodal imaging nanoparticles. This grafting was performed in a regio-selective way to preserve TEG4 activity and the avidity of the nanoparticles was studied with respect to the number of grafted antibodies. Subsequently, taking advantage of the nanoparticle bimodality, both near infrared fluorescence and magnetic resonance imaging of the atheroma plaque were performed in the ApoE−/− mouse model. Here we describe the design of the targeted nanoparticles, and a quantification method for their detection in mice, both ex vivo and in vivo, highlighting their value as a potential diagnosis agent.
Databáze: OpenAIRE
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