Effect of Dynamic Culture and Periodic Compression on Human Mesenchymal Stem Cell Proliferation and Chondrogenesis
| Autor: | Ting Guo, John P. Fisher, Bao-Ngoc B. Nguyen, Adam H. Hsieh, Jesse K. Placone, Addison S. Goodley, Casey G. Lim, Xuan Xiao, Li Yu, Kimberly M. Ferlin |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Compressive Strength Population Biomedical Engineering Type II collagen Cell Culture Techniques Article Extracellular matrix 03 medical and health sciences Humans Mesenchymal stem cell proliferation education Aggrecan Cell Proliferation education.field_of_study Chemistry Mesenchymal stem cell Mesenchymal Stem Cells Chondrogenesis Antigens Differentiation Cell biology 030104 developmental biology Gene Expression Regulation Cell culture Biomedical engineering |
| Popis: | We have recently developed a bioreactor that can apply both shear and compressive forces to engineered tissues in dynamic culture. In our system, alginate hydrogel beads with encapsulated human mesenchymal stem cells (hMSCs) were cultured under different dynamic conditions while subjected to periodic, compressive force. A customized pressure sensor was developed to track the pressure fluctuations when shear forces and compressive forces were applied. Compared to static culture, dynamic culture can maintain a higher cell population throughout the study. With the application of only shear stress, qRT-PCR and immunohistochemistry revealed that hMSCs experienced less chondrogenic differentiation than the static group. The second study showed that chondrogenic differentiation was enhanced by additional mechanical compression. After 14 days, alcian blue staining showed more extracellular matrix formed in the compression group. The upregulation of the positive chondrogenic markers such as Sox 9, aggrecan, and type II collagen were demonstrated by qPCR. Our bioreactor provides a novel approach to apply mechanical forces to engineered cartilage. Results suggest that a combination of dynamic culture with proper mechanical stimulation may promote efficient progenitor cell expansion in vitro, thereby allowing the culture of clinically relevant articular chondrocytes for the treatment of articular cartilage defects. |
| Databáze: | OpenAIRE |
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