Interplay of RhoA and mechanical forces in collective cell migration driven by leader cells
| Autor: | Sylvie Coscoy, François Amblard, A. Buguin, Myriam Reffay, Benoit Ladoux, Jacques Camonis, Pascal Silberzan, Olivier Cochet-Escartin, Maria Carla Parrini |
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| Přispěvatelé: | Physico-Chimie-Curie (PCC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Université Pierre et Marie Curie - Paris 6 (UPMC), CNRS UMR 7057 - Laboratoire Matières et Systèmes Complexes (MSC) (MSC), Centre National de la Recherche Scientifique (CNRS), Unité de génétique et biologie des cancers (U830), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM), Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Jacques Monod (IJM (UMR_7592)), Mechanobiology Institute [Singapore] (MBI), National University of Singapore (NUS), C'nano Ile de France, Association Christelle Bouillot, Association pour la Recherche sur le Cancer, Labex CelTisPhyBio, Institut Curie Programme Incitatif et Coopératif 'Physique de la Cellule', Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Matière et Systèmes Complexes (MSC), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Physico-Chimie-Curie ( PCC ), Centre National de la Recherche Scientifique ( CNRS ) -INSTITUT CURIE-Université Pierre et Marie Curie - Paris 6 ( UPMC ), CNRS UMR 7057 - Laboratoire Matières et Systèmes Complexes (MSC) ( MSC ), Centre National de la Recherche Scientifique ( CNRS ), Unité de génétique et biologie des cancers ( U830 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut Curie-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Matière et Systèmes Complexes ( MSC ), Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Jacques Monod ( IJM ), Mechanobiology Institute [Singapore] ( MBI ), National University of Singapore ( NUS ) |
| Rok vydání: | 2014 |
| Předmět: |
rac1 GTP-Binding Protein
RHOA MESH: Fluorescence Resonance Energy Transfer GTPase Madin Darby Canine Kidney Cells MESH: Dogs 0302 clinical medicine MESH : Dogs MESH : Protein Transport Cell Movement Fluorescence Resonance Energy Transfer MESH : Cell Movement MESH: Animals Pseudopodia MESH: Cell Movement Physics 0303 health sciences Tractive force biology Biomechanical Phenomena Cell biology Actin Cytoskeleton Protein Transport MESH: rhoA GTP-Binding Protein MESH: Protein Transport MESH: Biomechanical Phenomena [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Cell Adhesion Mechanical traction MESH : rac1 GTP-Binding Protein 03 medical and health sciences Dogs Cell Adhesion Animals Cell adhesion MESH : Biomechanical Phenomena MESH : rhoA GTP-Binding Protein 030304 developmental biology MESH : Madin Darby Canine Kidney Cells [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology MESH: rac1 GTP-Binding Protein Collective cell migration MESH: Madin Darby Canine Kidney Cells Cell Biology MESH : Cell Adhesion Multicellular organism MESH : Fluorescence Resonance Energy Transfer MESH : Actin Cytoskeleton MESH: Pseudopodia MESH : Pseudopodia biology.protein MESH : Animals MESH: Actin Cytoskeleton rhoA GTP-Binding Protein 030217 neurology & neurosurgery |
| Zdroj: | Nature Cell Biology Nature Cell Biology, Nature Publishing Group, 2014, 16 (3), pp.217-23. ⟨10.1038/ncb2917⟩ Nature Cell Biology, 2014, 16 (3), pp.217-23. ⟨10.1038/ncb2917⟩ Nature Cell Biology, Nature Publishing Group, 2014, 16 (3), pp.217-23. 〈10.1038/ncb2917〉 |
| ISSN: | 1476-4679 1465-7392 |
| DOI: | 10.1038/ncb2917 |
| Popis: | International audience; The leading front of a collectively migrating epithelium often destabilizes into multicellular migration fingers where a cell initially similar to the others becomes a leader cell while its neighbours do not alter. The determinants of these leader cells include mechanical and biochemical cues, often under the control of small GTPases. However, an accurate dynamic cartography of both mechanical and biochemical activities remains to be established. Here, by mapping the mechanical traction forces exerted on the surface by MDCK migration fingers, we show that these structures are mechanical global entities with the leader cells exerting a large traction force. Moreover, the spatial distribution of RhoA differential activity at the basal plane strikingly mirrors this force cartography. We propose that RhoA controls the development of these fingers through mechanical cues: the leader cell drags the structure and the peripheral pluricellular acto-myosin cable prevents the initiation of new leader cells. |
| Databáze: | OpenAIRE |
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