A critical role for IL-12 in CCR5 induction on T cell receptor-triggered mouse CD4+ and CD8+ T cells
| Autor: | Ping Gao, Michio Tomura, Woong Ryeon Park, Hiromi Fujiwara, Toshiyuki Hamaoka, Masayuki Iwasaki, Chigusa Nakajima, Takao Mukai |
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| Rok vydání: | 2001 |
| Předmět: |
CD4-Positive T-Lymphocytes
Receptors CCR5 viruses T cell Immunology Receptors Antigen T-Cell Fluorescent Antibody Technique C-C chemokine receptor type 6 CD8-Positive T-Lymphocytes CCR8 Biology CCL5 Interferon-gamma Mice medicine Animals Immunology and Allergy Cytotoxic T cell Calcium Signaling RNA Messenger Chemokine CCL4 Cells Cultured CXCL16 Mice Inbred BALB C virus diseases Macrophage Inflammatory Proteins Interleukin-12 Molecular biology Up-Regulation Cell biology medicine.anatomical_structure XCL2 Calcium CC chemokine receptors |
| Zdroj: | European Journal of Immunology. 31:2411-2420 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/1521-4141(200108)31:8<2411::aid-immu2411>3.0.co;2-y |
| Popis: | Despite increasing evidence for the role of the chemokine system in leukocyte trafficking, the mechanism underlying the induction of chemokine receptors is poorly understood. Here, we investigated how CCR5, a chemokine receptor implicated in T cell migration to inflammatory sites, is induced in the T cell. CCR5 mRNA was hardly detected in resting T cells and marginally induced following T cell receptor (TCR) stimulation. However, TCR-triggered T cells expressed IL-12 receptor, and stimulation with recombinant IL-12 resulted in high levels of CCR5 expression on both CD4(+) and CD8(+) T cells. In contrast, IL-2 failed to up-regulate CCR5 expression. The effect of IL-12 was selective to CCR5 because IL-12 did not up-regulate CXCR3 expression. Surface expression of CCR5 was shown by staining with anti-CCR5 monoclonal antibody. Stimulation of these CCR5-positive T cells with the relevant chemokine MIP-1 alpha elicited Ca(2+) influx, showing that IL-12-induced CCR5 is functional. These results indicate a critical role for IL-12 in the induction of CCR5 on TCR-triggered T cells. |
| Databáze: | OpenAIRE |
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