rSK1 in Rat Neurons: A Controller of Membrane rSK2?
| Autor: | Pankaj Sah, Li Xu, Angelo Tedoldi, Petra L. Sedlak, Eleonora Autuori, Margreet C. Ridder |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Small-Conductance Calcium-Activated Potassium Channels Cognitive Neuroscience Neuroscience (miscellaneous) Hippocampus Membrane Potentials lcsh:RC321-571 SK channel 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine SK3 excitability Animals Homomeric Rats Wistar lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Ion channel Original Research Neurons Chemistry Afterhyperpolarization Potassium channel Sensory Systems Rats Cell biology Protein Transport Cytosol 030104 developmental biology calcium activated K+ channels (KCa1–KCa5) Cytoplasm Neuroscience 030217 neurology & neurosurgery spike frequency adaptation potassium channel afterhyperpolarization |
| Zdroj: | Frontiers in Neural Circuits Frontiers in Neural Circuits, Vol 13 (2019) |
| ISSN: | 1662-5110 |
| DOI: | 10.3389/fncir.2019.00021 |
| Popis: | In mammalian neurons, small conductance calcium-activated potassium channels (SK channels) are activated by calcium influx and contribute to the afterhyperpolarization (AHP) that follows action potentials. Three types of SK channel, SK1, SK2 and SK3 are recognized and encoded by separate genes that are widely expressed in overlapping distributions in the mammalian brain. Expression of the rat genes, rSK2 and rSK3 generates functional ion channels that traffic to the membrane as homomeric and heteromeric complexes. However, rSK1 is not trafficked to the plasma membrane, appears not to form functional channels, and the role of rSK1 in neurons is not clear. Here, we show that rSK1 co-assembles with rSK2. rSK1 is not trafficked to the membrane but is retained in a cytoplasmic compartment. When rSK2 is present, heteromeric rSK1-rSK2 channels are also retained in the cytosolic compartment, reducing the total SK channel content on the plasma membrane. Thus, rSK1 appears to act as chaperone for rSK2 channels and expression of rSK1 may control the level of functional SK current in rat neurons. |
| Databáze: | OpenAIRE |
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