Association Between microRNA-125a rs12976445 CT Polymorphism and 18F-Fluorodeoxyglucose (18FDG) Uptake: Clinical and Metabolic Response in Patients with Non-Small Cell Lung Cancer
| Autor: | Jinjin Zhou, Wenhua Guan, Zhina Zang, Yan Han, Diansen Chen, Zhan Shi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Untranslated region Male Vascular Endothelial Growth Factor A Lung Neoplasms Biology Transfection Polymorphism Single Nucleotide 03 medical and health sciences chemistry.chemical_compound Fluorodeoxyglucose F18 Genes Reporter Clinical Research Carcinoma Non-Small-Cell Lung microRNA medicine Humans Luciferase Genetic Predisposition to Disease Lung cancer Luciferases Genetic Association Studies Demography Regulation of gene expression Messenger RNA Base Sequence General Medicine Middle Aged medicine.disease Molecular biology Vascular endothelial growth factor Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology chemistry A549 Cells Hepatocellular carcinoma Female Basal Metabolism |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| Popis: | BACKGROUND MicroRNA-125a (miR-125a) has been involved with many diseases, such as hepatocellular carcinoma and inflammation. In this study, we aimed to investigate the molecular mechanism, including the potential regulator and signaling pathways, of vascular endothelial growth factor (VEGF). MATERIAL AND METHODS We divided the participants into 3 groups by rs12976445 genotype and performed chi-square tests to evaluate the differences between CC and CT+TT groups for sex, age, grading, pT category, metastases, and fludeoxyglucose F18 injection (18FDG) metabolism. RESULTS We found all variables to be statistically significant. We searched the miRNA database online (www.mirdb.org) with the "seed sequence" located within the 3-prime untranslated region (3' UTR) of the target gene and then validated VEGF to be the direct gene via luciferase reporter assay system. We also established the negative regulatory relationship between MiR-125a and VEGF by studying the relative luciferase activity. We conducted real-time polymerase chain reaction and Western blot analysis to study the mRNA and protein expression level of VEGF among different groups (CC=18, CT=8, TT=3) or cells treated with scramble control, miR-125a mimics, VEGF RNA, and MiR-125a inhibitors. CONCLUSIONS We validated the negative regulatory relationship between MiR-125a and VEGF and found that rs12976445 may function as a biomarker to predict metabolism of 18FDG. |
| Databáze: | OpenAIRE |
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