Bioactivation Treatment with Mixed Acid and Heat on Titanium Implants Fabricated by Selective Laser Melting Enhances Preosteoblast Cell Differentiation
| Autor: | Tatsuya Kakutani, Kitagaki Hisashi, Hiroyuki Nakano, Yoichiro Nakajima, Tomiharu Matsushita, Hiroaki Takadama, Kazuya Inoue, Takaaki Ueno, Morihiro Ito, Seine A. Shintani, Phuc Thi Minh Le, Seiji Yamaguchi, Shuntaro Terauchi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
General Chemical Engineering Cellular differentiation 0206 medical engineering chemistry.chemical_element 02 engineering and technology Article Osseointegration Apatite lcsh:Chemistry Surface roughness General Materials Science titanium Selective laser melting cell culture 021001 nanoscience & nanotechnology 020601 biomedical engineering chemistry Chemical engineering lcsh:QD1-999 visual_art selective laser melting visual_art.visual_art_medium Wetting 0210 nano-technology Titanium acid treatment apatite formation |
| Zdroj: | Nanomaterials Volume 11 Issue 4 Nanomaterials, Vol 11, Iss 987, p 987 (2021) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano11040987 |
| Popis: | Selective laser melting (SLM) is a promising technology capable of producing individual characteristics with a high degree of surface roughness for implants. These surfaces can be modified so as to increase their osseointegration, bone generation and biocompatibility, features which are critical to their clinical success. In this study, we evaluated the effects on preosteoblast proliferation and differentiation of titanium metal (Ti) with a high degree of roughness (Ra = 5.4266 ± 1.282 µm) prepared by SLM (SLM-Ti) that was also subjected to surface bioactive treatment by mixed acid and heat (MAH). The results showed that the MAH treatment further increased the surface roughness, wettability and apatite formation capacity of SLM-Ti, features which are useful for cell attachment and bone bonding. Quantitative measurement of osteogenic-related gene expression by RT-PCR indicated that the MC3T3-E1 cells on the SLM-Ti MAH surface presented a stronger tendency towards osteogenic differentiation at the genetic level through significantly increased expression of Alp, Ocn, Runx2 and Opn. We conclude that bio-activated SLM-Ti enhanced preosteoblast differentiation. These findings suggest that the mixed acid and heat treatment on SLM-Ti is promising method for preparing the next generation of orthopedic and dental implants because of its apatite formation and cell differentiation capability. |
| Databáze: | OpenAIRE |
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