Role of Na+ transporters HKT1;1 and HKT1;2 in tomato salt tolerance. I. Function loss of cheesmaniae alleles in roots and aerial parts
| Autor: | Paloma González-Fernández, M. J. Asins, José A. Traverso, Jesús Espinosa, Emilio Jaime-Fernández, Andrés Belver, María Remedios Romero-Aranda |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), European Commission, Universidad de Granada, Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2021 |
| Předmět: |
Salinity
F61 Plant physiology - Nutrition Physiology Vegetative reproduction 1 and ScHKT1 Fruit yield and quality Plant Science Tomato Tomatoes Solanum cheesmaniae ScHKT1 Genetics 2 genes F40 Plant ecology education K+ and Na+ homeostasis education.field_of_study Grafting F62 Plant physiology - Growth and development Chemistry Fruit yield fungi food and beverages Xylem Q01 Food science and technology F60 Plant physiology and biochemistry Quality ScHKT1.1 and ScHKT1 Horticulture Phloem Rootstock Homeostasis F30 Plant genetics and breeding |
| Zdroj: | electronico ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias instname Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 0981-9428 |
| Popis: | We wish to thank Elena Sanchez Romero (EEZ-CSIC) for her technical assistance, the Scientific Instrumentation Service at EEZ-CSIC, Granada, for their ICP-OES mineral analysis and Michael O'Shea for proofreading the manuscript. The study was funded by grant AGL201782452-C2-1R (A.B.) and grant AGL2017-82452-C2-2R (M.J.A.), both from the Spanish Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion, MCIN/AEI/10.13039/501100011033, and FEDER "Una manera de hacer Europa", as well as grant ACCESP2018 (J.A.T.) from the University of Granada. J.E. was supported by a JAE Intro-CSIC grant, JAEINT_19_00566. We analyzed the physiological impact of function loss on cheesmaniae alleles at the HKT1;1 and HKT1;2 loci in the roots and aerial parts of tomato plants in order to determine the relative contributions of each locus in the different tissues to plant Na+/K+ homeostasis and subsequently to tomato salt tolerance. We generated different reciprocal rootstock/scion combinations with non-silenced, single RNAi-silenced lines for ScHKT1;1 and ScHKT1;2, as well as a silenced line at both loci from a near isogenic line (NIL14), homozygous for the Solanum cheesmaniae haplotype containing both HKT1 loci and subjected to salinity under natural greenhouse conditions. Our results show that salt treatment reduced vegetative growth and altered the Na+/K+ ratio in leaves and flowers; negatively affecting fruit production, particularly in graft combinations containing single silenced ScHKT1;2- and double silenced ScHKT1;1/ScHKT1;2 lines when used as scion. We concluded that the removal of Na+ from the xylem by ScHKT1;2 in the aerial part of the plant can have an even greater impact than that on Na+ homeostasis at the root level under saline conditions. Also, ScHKT1;1 function loss in rootstock greatly reduced the Na+/K+ ratio in leaf and flower tissues, minimized yield loss under salinity. Our results suggest that, in addition to xylem Na+ unloading, ScHKT1;2 could also be involved in Na+ uploading into the phloem, thus promoting Na+ recirculation from aerial parts to the roots. This recirculation of Na+ to the roots through the phloem could be further favoured by ScHKT1;1 silencing at these roots. Spanish Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion AGL2017-82452-C2-1R AGL2017-82452-C2-2R FEDER "Una manera de hacer Europa" MCIN/AEI/10.13039/501100011033 University of Granada ACCESP2018 JAE Intro-CSIC grant JAEINT_19_00566 |
| Databáze: | OpenAIRE |
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