Expression of G2A, a Receptor for Lysophosphatidylcholine, by Macrophages in Murine, Rabbit, and Human Atherosclerotic Plaques
| Autor: | Yoshiyuki Rikitake, Junya Ejiri, Nobutaka Inoue, Kenji Iwai, Seiichi Kobayashi, Ken-ichi Hirata, Masanori Ozaki, Tomoya Yamashita, Masashi Shiomi, Mitsuhiro Yokoyama, Seinosuke Kawashima, Hiroshi Itoh |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Umbilical Veins
Pathology medicine.medical_specialty Apolipoprotein B Endothelium Aorta Thoracic Cell Cycle Proteins Receptors Cell Surface Coronary Artery Disease GPR132 Cardiovascular System complex mixtures Monocytes Muscle Smooth Vascular Receptors G-Protein-Coupled Pathogenesis Jurkat Cells Mice chemistry.chemical_compound Apolipoproteins E parasitic diseases Tumor Cells Cultured medicine Animals Humans Macrophage Receptor Cells Cultured Mice Knockout biology Macrophages Lysophosphatidylcholines Coronary Vessels Lysophosphatidylcholine medicine.anatomical_structure chemistry Macrophages Peritoneal biology.protein Immunohistochemistry Endothelium Vascular Rabbits Cardiology and Cardiovascular Medicine |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 22:2049-2053 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000040598.18570.54 |
| Popis: | Objective— Lysophosphatidylcholine (LPC), a major phospholipid component of oxidized low density lipoprotein, has been demonstrated to induce multiple functional alterations of vasculature that are potentially involved in atherosclerosis. Recently, an orphan G-protein-coupled receptor, G2A, has been identified as a high-affinity receptor for LPC. Although it has been demonstrated that G2A is expressed predominantly in lymphoid tissues and lymphocytes, there are no reports to determine whether G2A is expressed in atherosclerotic lesions and cardiovascular cells. Methods and Results— Immunohistochemistry with an anti-G2A antibody revealed that G2A was expressed predominantly by macrophages within atherosclerotic lesions at the aortic root of apolipoprotein E-deficient mice and the thoracic aortas of Watanabe heritable hyperlipidemic rabbits. In atherosclerotic plaques of human coronary arterial specimens, G2A was expressed by macrophages within the lipid-rich plaques, whereas no immunoreactivity of G2A was observed in fibrous plaques where macrophages did not exist. Reverse transcription-polymerase chain reaction analysis demonstrated that G2A mRNA was highly expressed in human and murine monocytes/macrophages. The expression of G2A protein was detected in human and murine monocytes/macrophages by immunoblotting. Conclusions— These findings demonstrate that monocytes/macrophages abundantly express G2A and suggest that G2A may play a role in the formation and progression of atherosclerotic lesions. |
| Databáze: | OpenAIRE |
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