Gnotobiotic and Conventional Mouse Systems to Support Microbiota Based Studies
| Autor: | Richard Lavin, Lynn Bry, Vladimir Yeliseyev, Nicholas DiBenedetto, Mary L. Delaney |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Background information Microbiological Techniques Microbiota 030106 microbiology Immunology Causal effect Sterilization Computational biology Disease Biology Article 03 medical and health sciences Feces Mice 030104 developmental biology In vivo Diagnostic biomarker Animals Germ-Free Life Humans Microbiome |
| Zdroj: | Current protocols in immunology. 121(1) |
| ISSN: | 1934-368X |
| Popis: | Animal models are essential to dissect host-microbiota interactions that impact health and the development of disease. In addition to providing pre-clinical models for the development of novel therapeutics and diagnostic biomarkers, mouse systems actively support microbiome studies by defining microbial contributions to normal development and homeostasis, and as well as their role in promoting diseases such as inflammatory auto-immune diseases, diabetes, metabolic syndromes, and susceptibilities to infectious agents. Mice provide a genetically tenable host that can be reared under gnotobiotic (germfree) conditions, allowing colonization studies with human or mouse-origin defined or complex microbial communities to define specific in vivo effects. The protocols and background information detail key aspects to consider in designing host-microbiome experiments with mouse models, and to develop robust systems that leverage gnotobiotic mice, microbial consortia, and specific environmental perturbations to identify causal effects in vivo. |
| Databáze: | OpenAIRE |
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