Practical considerations in the use of polarized light microscopy in the analysis of the collagen network in articular cartilage
| Autor: | Jarno Rieppo, Jukka S. Jurvelin, Ilkka Kiviranta, Helminen Hj, Mika M. Hyttinen, Jarmo Hallikainen |
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| Rok vydání: | 2008 |
| Předmět: |
Cartilage
Articular Polarized light microscopy Birefringence Histology Materials science business.industry Cartilage Characterization (materials science) Fibril-Associated Collagens Medical Laboratory Technology Optics medicine.anatomical_structure Tissue engineering Microscopy Collagen network Articular cartilage repair medicine Animals Cattle Collagen Microscopy Polarization Anatomy business Instrumentation |
| Zdroj: | Microscopy Research and Technique. 71:279-287 |
| ISSN: | 1097-0029 1059-910X |
| DOI: | 10.1002/jemt.20551 |
| Popis: | Polarized light microscopy is a traditional method for visualizing the collagen network architecture of articular cartilage. Articular cartilage repair and tissue engineering studies have raised new demands for techniques capable of quantitative characterization of the scar and repair tissues, including properties of the collagen network. Modern polarized light microscopy can be used to measure collagen fibril orientation, parallelism, and birefringence. New commercial instruments are computer controlled and the measurements are easy to perform. However, often the interpretation of results causes difficulties, even errors, because the theoretical aspects of the technique are demanding. The aim of this study was to describe the instrumentation and properties of a modern polarized light microscope, to point out some sources of error in the interpretation of the results, and to recall the theoretical background of the polarized light microscopy. |
| Databáze: | OpenAIRE |
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