An improved experimental model for peripheral neuropathy in rats
| Autor: | Quintino Moura Dias, Rafael Sobrano Fais, A.C. Rossaneis, William Alves do Prado |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Pain Threshold medicine.medical_specialty Time Factors Physiology medicine.medical_treatment Immunology Biophysics Hindlimb Biochemistry Chronic constriction injury Threshold of pain Medicine Animals DOR General Pharmacology Toxicology and Pharmaceutics Rats Wistar Ligature lcsh:QH301-705.5 Pain Measurement Peripheral nerve lesion lcsh:R5-920 business.industry General Neuroscience Tribromoethanol Biomedical Sciences Peripheral Nervous System Diseases Cell Biology General Medicine medicine.disease Sciatic Nerve Surgery Neuropathy Disease Models Animal Peripheral neuropathy Spinal nerve ligation lcsh:Biology (General) Hyperalgesia Anesthesia Reflex Sciatic nerve medicine.symptom business Chronic pain model lcsh:Medicine (General) |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 46, Iss 3, Pp 253-256 (2013) Brazilian Journal of Medical and Biological Research Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Medical and Biological Research v.46 n.3 2013 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 3, Pages: 253-256, Published: 15 MAR 2013 |
| Popis: | A modification of the Bennett and Xie chronic constriction injury model of peripheral painful neuropathy was developed in rats. Under tribromoethanol anesthesia, a single ligature with 100% cotton glace thread was placed around the right sciatic nerve proximal to its trifurcation. The change in the hind paw reflex threshold after mechanical stimulation observed with this modified model was compared to the change in threshold observed in rats subjected to the Bennett and Xie or the Kim and Chung spinal ligation models. The mechanical threshold was measured with an automated electronic von Frey apparatus 0, 2, 7, and 14 days after surgery, and this threshold was compared to that measured in sham rats. All injury models produced significant hyperalgesia in the operated hind limb. The modified model produced mean ± SD thresholds in g (19.98 ± 3.08, 14.98 ± 1.86, and 13.80 ± 1.00 at 2, 7, and 14 days after surgery, respectively) similar to those obtained with the spinal ligation model (20.03 ± 1.99, 13.46 ± 2.55, and 12.46 ± 2.38 at 2, 7, and 14 days after surgery, respectively), but less variable when compared to the Bennett and Xie model (21.20 ± 8.06, 18.61 ± 7.69, and 18.76 ± 6.46 at 2, 7, and 14 days after surgery, respectively). The modified method required less surgical skill than the spinal nerve ligation model. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|