Neuroprotective Effects of Chalcones from Myracrodruon urundeuva on 6-Hydroxydopamine-Induced Cytotoxicity in Rat Mesencephalic Cells
| Autor: | Manoel Odorico de Moraes, R.A. Oliveira, Hélio V. Nobre-Junior, Marcelle A. S. Nogueira, F.D. Maia, Geanne Matos de Andrade, Glauce Socorro de Barros Viana, Mary Anne Medeiros Bandeira |
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| Rok vydání: | 2008 |
| Předmět: |
Programmed cell death
animal structures Necrosis Tyrosine 3-Monooxygenase Anacardiaceae Dopamine medicine.disease_cause Thiobarbituric Acid Reactive Substances Biochemistry Neuroprotection Cellular and Molecular Neuroscience chemistry.chemical_compound Chalcones Mesencephalon medicine TBARS Animals Viability assay Rats Wistar Oxidopamine Cells Cultured Nitrites Neurons Cell Death Cytotoxins Chemistry Acridine orange General Medicine Immunohistochemistry Molecular biology Rats Oxidative Stress Neuroprotective Agents nervous system Apoptosis Lipid Peroxidation medicine.symptom Oxidative stress |
| Zdroj: | Neurochemical Research. 34:1066-1075 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-008-9876-5 |
| Popis: | In the present work, we showed that a chalcone-enriched fraction (CEF) isolated from the stem bark of a Brazilian medicinal plant, Myracrodruon urundeuva, presents neuroprotective actions on 6-hydroxydopamine (6-OHDA)-induced neuronal cell death, in rat mesencephalic cells. In the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] assay, which is an index of cell viability, CEF (1-100 microg/ml) reversed in a concentration-dependent manner the 6-OHDA-induced cell death. While cells exposed to 6-OHDA (40 microM) showed an increased concentration of thiobarbituric acid reactive substances (TBARS), the pretreatment with CEF (10-100 microg/ml) significantly decreased the 6-OHDA-induced TBARS formation, indicative of a neuroprotection against lipoperoxidation. Furthermore, the drastic increase of nitrite levels induced by 6-OHDA, indicative of nitric oxide formation and free radicals production, was prevented by CEF. Double staining with acridine orange/ethidium bromide showed that cultures exposed to 6-OHDA (40 and 200 microM) presented an increase of apoptotic and necrotic cell numbers in a concentration-dependent manner. CEF (100 microg/ml) protected cells from apoptosis and necrosis and increased number of cells presenting a normal morphology. The immunohistochemical analysis for tyrosine hydroxylase (TH) positive neurons indicated that 6-OHDA (40 and 200 microM) caused a concentration-dependent loss of TH+ and TH- neurons. CEF protected both cells types from 6-OHDA-induced cell death. All together, our results demonstrated neuroprotective effects of chalcones, which are able to reduce oxidative stress and apoptotic injury caused by 6-OHDA. Our findings suggest that chalcones could provide benefits, along with other therapies, in neurodegenerative injuries, such as Parkinson's disease. |
| Databáze: | OpenAIRE |
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