Surface microstructures on planar substrates and textile fibers guide neurite outgrowth: a scaffold solution to push limits of critical nerve defect regeneration?
| Autor: | Ursina Tobler, Thomas Lehnert, Manuel Wiesli, Thomas Osterwalder, Arie Bruinink, Abhay Pandit, Li Yao, Stefan Weigel |
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| Rok vydání: | 2012 |
| Předmět: |
Polymers
Biocompatible Materials Chick Embryo axonal growth Planar Coating Morphogenesis Composite material Silicon oxide Microstructure schwann-cells Neurons Multidisciplinary geography.geographical_feature_category Tissue Scaffolds Neuronal Morphology Chemistry Anatomy Axon Guidance Spinal Cord Ridge artificial microstructures Medicine Material by Structure Polyimide growth cones Research Article Neurite Science Materials Science peripheral-nerve engineering.material in-vitro topographical control neuronal cells Biomaterials Developmental Neuroscience Neurites Animals Regeneration Growth cone Biology geography Tissue Engineering behavior Amorphous solid Nerve Regeneration contact guidance Cellular Neuroscience engineering Developmental Biology Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 12, p e50714 (2012) |
| ISSN: | 1932-6203 |
| Popis: | The treatment of critical size peripheral nerve defects represents one of the most serious problems in neurosurgery. If the gap size exceeds a certain limit, healing can't be achieved. Connection mismatching may further reduce the clinical success. The present study investigates how far specific surface structures support neurite outgrowth and by that may represent one possibility to push distance limits that can be bridged. For this purpose, growth cone displacement of fluorescent embryonic chicken spinal cord neurons was monitored using time-lapse video. In a first series of experiments, parallel patterns of polyimide ridges of different geometry were created on planar silicon oxide surfaces. These channel-like structures were evaluated with and without amorphous hydrogenated carbon (a-C:H) coating. In a next step, structured and unstructured textile fibers were investigated. All planar surface materials (polyimide, silicon oxide and a-C:H) proved to be biocompatible, i.e. had no adverse effect on nerve cultures and supported neurite outgrowth. Mean growth cone migration velocity measured on 5 minute base was marginally affected by surface structuring. However, surface structure variability, i.e. ridge height, width and inter-ridge spacing, significantly enhanced the resulting net velocity by guiding the growth cone movement. Ridge height and inter-ridge distance affected the frequency of neurites crossing over ridges. Of the evaluated dimensions ridge height, width, and inter-ridge distance of respectively 3, 10, and 10 mu m maximally supported net axon growth. Comparable artificial grooves, fabricated onto the surface of PET fibers by using an excimer laser, showed similar positive effects. Our data may help to further optimize surface characteristics of artificial nerve conduits and bioelectronic interfaces. |
| Databáze: | OpenAIRE |
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