Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody
| Autor: | D L Brawner, J E Cutler |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Antigens
Fungal Immunology Fluorescent Antibody Technique Microbiology Epitope Absorption Candida tropicalis Antigen-Antibody Reactions Epitopes Mice Agglutinin Antigen Candida albicans Animals Mice Inbred BALB C biology Candidiasis Antibodies Monoclonal biology.organism_classification Precipitin Molecular biology Corpus albicans Culture Media Infectious Diseases Agglutinins Antigens Surface biology.protein Parasitology Antibody Research Article |
| Zdroj: | Infection and immunity. 43(3) |
| ISSN: | 0019-9567 |
| Popis: | The expression of a surface immunodeterminant of Candida albicans was investigated with an agglutinating immunoglobulin M monoclonal antibody. The 96 strains of C. albicans tested, of which 76% were recent clinical isolates, were capable of expressing the antigen. The antigen was also produced by strains of Candida tropicalis and Torulopsis glabrata, but not by other yeast species. Expression of the surface immunodeterminant in C. albicans varied as a function of growth as indicated by agglutinin reactions and indirect immunofluorescence tests. When yeast cells were tested with the agglutinin, three patterns of reactivity were observed. In general, cells in the early logarithmic phase were less reactive than cells in the mid-logarithmic phase. Antigen expression, as determined by agglutinin reactivity, was also influenced by the nutritional composition of the growth medium, and in general, cells from broth cultures were usually more reactive than cells grown on solid media. The antigen was solubilized from the cell surface of C. albicans by hot 1 M NaCl. These water-soluble extracts were capable of binding antibody, and a single precipitin band formed when soluble antigen was reacted with the monoclonal antibody in an Ouchterlony double-diffusion test. Whole cell preparations and hot NaCl extracts from yeast strains which did not agglutinate when mixed with the antibody also did not absorb the agglutinin from solution. These data indicate that the expression of surface antigens on C. albicans is a dynamic process which may be influenced by a number of environmental factors. The use of monoclonal antibodies may allow characterization of surface antigens presented to the host during candidiasis. |
| Databáze: | OpenAIRE |
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